Field measurements of genotype by environment interaction for fitness caused by spontaneous mutations in Arabidopsis thaliana

Title:
Field measurements of genotype by environment interaction for fitness caused by spontaneous mutations in Arabidopsis thaliana
Authors:
Roles, Angela J.; Rutter, Matthew T.; Dworkin, Ian; Fenster, Charles B.; Conner, Jeffrey K.
Abstract:
As the ultimate source of genetic diversity, spontaneous mutation is critical to the evolutionary process. The fitness effects of spontaneous mutations are almost always studied under controlled laboratory conditions rather than under the evolutionarily relevant conditions of the field. Of particular interest is the conditionality of new mutations—that is, is a new mutation harmful regardless of the environment in which it is found? In other words, what is the extent of genotype–environment interaction for spontaneous mutations? We studied the fitness effects of 25 generations of accumulated spontaneous mutations in Arabidopsis thaliana in two geographically widely separated field environments, in Michigan and Virginia. At both sites, mean total fitness of mutation accumulation lines exceeded that of the ancestors, contrary to the expected decrease in the mean due to new mutations but in accord with prior work on these MA lines. We observed genotype–environment interactions in the fitness effects of new mutations, such that the effects of mutations in Michigan were a poor predictor of their effects in Virginia and vice versa. In particular, mutational variance for fitness was much larger in Virginia compared to Michigan. This strong genotype–environment interaction would increase the amount of genetic variation maintained by mutation-selection balance.
Citation:
Roles, Angela J., Matthew T. Rutter, Ian Dworkin, et al. 2016. "Field measurements of genotype by environment interaction for fitness caused by spontaneous mutations in Arabidopsis thaliana." Evolution 70(5): 1039-1050.
Publisher:
Wiley for the Society for the Study of Evolution
DATE ISSUED:
2016-05
Department:
Biology
Type:
Article
PUBLISHED VERSION:
10.1111/evo.12913
PERMANENT LINK:
http://hdl.handle.net/11282/617322

Full metadata record

DC FieldValue Language
dc.contributor.authorRoles, Angela J.en
dc.contributor.authorRutter, Matthew T.en
dc.contributor.authorDworkin, Ianen
dc.contributor.authorFenster, Charles B.en
dc.contributor.authorConner, Jeffrey K.en
dc.date.accessioned2016-07-21T12:41:40Z-
dc.date.available2016-07-21T12:41:40Z-
dc.date.issued2016-05-
dc.identifier.citationRoles, Angela J., Matthew T. Rutter, Ian Dworkin, et al. 2016. "Field measurements of genotype by environment interaction for fitness caused by spontaneous mutations in Arabidopsis thaliana." Evolution 70(5): 1039-1050.en
dc.identifier.issn0014-3820-
dc.identifier.urihttp://hdl.handle.net/11282/617322-
dc.description.abstractAs the ultimate source of genetic diversity, spontaneous mutation is critical to the evolutionary process. The fitness effects of spontaneous mutations are almost always studied under controlled laboratory conditions rather than under the evolutionarily relevant conditions of the field. Of particular interest is the conditionality of new mutations—that is, is a new mutation harmful regardless of the environment in which it is found? In other words, what is the extent of genotype–environment interaction for spontaneous mutations? We studied the fitness effects of 25 generations of accumulated spontaneous mutations in Arabidopsis thaliana in two geographically widely separated field environments, in Michigan and Virginia. At both sites, mean total fitness of mutation accumulation lines exceeded that of the ancestors, contrary to the expected decrease in the mean due to new mutations but in accord with prior work on these MA lines. We observed genotype–environment interactions in the fitness effects of new mutations, such that the effects of mutations in Michigan were a poor predictor of their effects in Virginia and vice versa. In particular, mutational variance for fitness was much larger in Virginia compared to Michigan. This strong genotype–environment interaction would increase the amount of genetic variation maintained by mutation-selection balance.en
dc.language.isoen_USen
dc.publisherWiley for the Society for the Study of Evolutionen
dc.identifier.doi10.1111/evo.12913-
dc.subject.departmentBiologyen_US
dc.titleField measurements of genotype by environment interaction for fitness caused by spontaneous mutations in Arabidopsis thalianaen_US
dc.typeArticleen
dc.identifier.journalEvolution: International Journal of Organic Evolutionen
dc.subject.keywordCrossing GEIen_US
dc.subject.keywordMutation accumulationen_US
dc.subject.keywordMutation-selection balanceen_US
dc.subject.keywordVariance GEIen_US
dc.identifier.volume70en_US
dc.identifier.issue5en_US
dc.identifier.startpage1039en_US
dc.rightsArchived with thanks to Evolutionen
All Items in The Five Colleges of Ohio Digital Repository are protected by copyright, with all rights reserved, unless otherwise indicated.