Expression analysis of Cdx2 and Pou5f1 in a marsupial, the stripe-faced dunnart, during early development

Title:
Expression analysis of Cdx2 and Pou5f1 in a marsupial, the stripe-faced dunnart, during early development
Authors:
Familari, Mary; Au, Phil Chi Khang; de Iongh, Robb U.; Cruz, Yolanda P.; Selwood, Lynne
Abstract:
The first lineage allocation during mouse development forms the trophectoderm and inner cell mass, in which Cdx2 and Pou5f1 display reciprocal expression. Yet Cdx2 is not required for trophectoderm specification in other mammals, such as the human, cow, pig, or in two marsupials, the tammar and opossum. The role of Cdx2 and Pou5f1 in the first lineage allocation of Sminthopsis macroura, the stripe-faced dunnart, is unknown. In this study, expression of Cdx2 and Pou5f1 during oogenesis, development from cleavage to blastocyst stages, and in the allocation of the first three lineages was analyzed for this dunnart. Cdx2 mRNA was present in late antral-stage oocytes, but not present again until Day 5.5. Pou5f1 mRNA was present from primary follicles to zygotes, and then expression resumed starting at the early unilaminar blastocyst stage. All cleavage stages and the pluriblast and trophoblast cells co-expressed CDX2 and POU5F1 proteins, which persisted until early stages of hypoblast formation. Hypoblast cells also show co-localisation of POU5F1 and CDX2 once they were allocated, and this persisted during their division and migration. Our studies suggest that CDX2, and possibly POU5F1, are maternal proteins, and that the first lineage to differentiate is the trophoblast, which differentiates to trophectoderm after shell loss one day before implantation. In the stripe-faced dunnart, cleavage cells, as well as trophoblast and pluriblast cells, are polarized, suggesting the continued presence of CDX2 in both lineages until late blastocyst stages may play a role in the formation and maintenance of polarity. Mol. Reprod. Dev. 83: 108–123, 2016. © 2015 Wiley Periodicals, Inc.
Citation:
Familari, Mary, Phil Chi Khang Au, Robb U. de longh, et al. 2016. "Expression analysis of Cdx2 and Pou5f1 in a marsupial, the stripe-faced dunnart, during early development." Molecular Reproduction and Development 83(2): 108-123.
Publisher:
Wiley
DATE ISSUED:
2016-03-10
Department:
Biology
Type:
Article
PUBLISHED VERSION:
10.1002/mrd.22597
PERMANENT LINK:
http://hdl.handle.net/11282/601133

Full metadata record

DC FieldValue Language
dc.contributor.authorFamilari, Maryen
dc.contributor.authorAu, Phil Chi Khangen
dc.contributor.authorde Iongh, Robb U.en
dc.contributor.authorCruz, Yolanda P.en
dc.contributor.authorSelwood, Lynneen
dc.date.accessioned2016-03-10T19:02:35Zen
dc.date.available2016-03-10T19:02:35Zen
dc.date.issued2016-03-10en
dc.identifier.citationFamilari, Mary, Phil Chi Khang Au, Robb U. de longh, et al. 2016. "Expression analysis of Cdx2 and Pou5f1 in a marsupial, the stripe-faced dunnart, during early development." Molecular Reproduction and Development 83(2): 108-123.en
dc.identifier.issn1040-452Xen
dc.identifier.urihttp://hdl.handle.net/11282/601133en
dc.description.abstractThe first lineage allocation during mouse development forms the trophectoderm and inner cell mass, in which Cdx2 and Pou5f1 display reciprocal expression. Yet Cdx2 is not required for trophectoderm specification in other mammals, such as the human, cow, pig, or in two marsupials, the tammar and opossum. The role of Cdx2 and Pou5f1 in the first lineage allocation of Sminthopsis macroura, the stripe-faced dunnart, is unknown. In this study, expression of Cdx2 and Pou5f1 during oogenesis, development from cleavage to blastocyst stages, and in the allocation of the first three lineages was analyzed for this dunnart. Cdx2 mRNA was present in late antral-stage oocytes, but not present again until Day 5.5. Pou5f1 mRNA was present from primary follicles to zygotes, and then expression resumed starting at the early unilaminar blastocyst stage. All cleavage stages and the pluriblast and trophoblast cells co-expressed CDX2 and POU5F1 proteins, which persisted until early stages of hypoblast formation. Hypoblast cells also show co-localisation of POU5F1 and CDX2 once they were allocated, and this persisted during their division and migration. Our studies suggest that CDX2, and possibly POU5F1, are maternal proteins, and that the first lineage to differentiate is the trophoblast, which differentiates to trophectoderm after shell loss one day before implantation. In the stripe-faced dunnart, cleavage cells, as well as trophoblast and pluriblast cells, are polarized, suggesting the continued presence of CDX2 in both lineages until late blastocyst stages may play a role in the formation and maintenance of polarity. Mol. Reprod. Dev. 83: 108–123, 2016. © 2015 Wiley Periodicals, Inc.en
dc.language.isoen_USen
dc.publisherWileyen
dc.identifier.doi10.1002/mrd.22597en
dc.subject.departmentBiologyen_US
dc.titleExpression analysis of Cdx2 and Pou5f1 in a marsupial, the stripe-faced dunnart, during early developmenten_US
dc.typeArticleen
dc.identifier.journalMolecular Reproduction and Developmenten
dc.subject.keywordHypoblasten_US
dc.subject.keywordLineage allocationen_US
dc.subject.keywordSminthopsis macrouraen_US
dc.subject.keywordTrophectodermen_US
dc.identifier.volume83en_US
dc.identifier.issue2en_US
dc.identifier.startpage108en_US
dc.rightsArchived with thanks to Molecular Reproduction and Developmenten
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