Mouse blastocyst immunosurgery with commercial antiserum to mouse erythrocytes

Title:
Mouse blastocyst immunosurgery with commercial antiserum to mouse erythrocytes
Authors:
Cruz, Yolanda P.; Treichel, Robin S.; Harsay, E.; Chi, K. D.
Abstract:
Immunosurgery is a useful technique for the isolation of inner cell masses from murine blastocysts. Conventionally, rabbit antisera made ad hoc against murine splenic or fetal cells or fibroblasts have been used as antibody sources. We investigated the feasibility of using commercially available rabbit antiserum to murine erythrocytes (anti-RBC) and compared it with rabbit antiserum generated ad hoc to murine L-cells (anti-L-cell). Our results indicate that anti-RBC is at least as effective as anti-L-cell serum for the immunosurgical isolation of inner cell masses, which became either miniblastocysts (later forming outgrowths) or embryoid bodies (undergoing ectoderm-endodermlike differentiation within 48 h). Because anti-RBC is commercially available, the technical modification described herein increases the accessibility of the immunosurgical protocol for the isolation of murine inner cell masses.
Citation:
Cruz, Y.P., R.S. Treichel, E. Harsay, and K.D. Chi. 1993. "Mouse blastocyst immunosurgery with commercial antiserum to mouse erythrocytes." In Vitro Cellular and Developmental Biology - Animal 29(8): 671-675.
Publisher:
Society for In-Vitro Biology / Springer Verlag
DATE ISSUED:
1993-08
Department:
Biology
Type:
Article
PUBLISHED VERSION:
10.1007/BF02634557
Additional Links:
http://link.springer.com/10.1007/BF02634557
PERMANENT LINK:
http://hdl.handle.net/11282/600494

Full metadata record

DC FieldValue Language
dc.contributor.authorCruz, Yolanda P.en
dc.contributor.authorTreichel, Robin S.en
dc.contributor.authorHarsay, E.en
dc.contributor.authorChi, K. D.en
dc.date.accessioned2016-03-02T14:28:56Zen
dc.date.available2016-03-02T14:28:56Zen
dc.date.issued1993-08en
dc.identifier.citationCruz, Y.P., R.S. Treichel, E. Harsay, and K.D. Chi. 1993. "Mouse blastocyst immunosurgery with commercial antiserum to mouse erythrocytes." In Vitro Cellular and Developmental Biology - Animal 29(8): 671-675.en
dc.identifier.issn0883-8364en
dc.identifier.urihttp://hdl.handle.net/11282/600494en
dc.description.abstractImmunosurgery is a useful technique for the isolation of inner cell masses from murine blastocysts. Conventionally, rabbit antisera made ad hoc against murine splenic or fetal cells or fibroblasts have been used as antibody sources. We investigated the feasibility of using commercially available rabbit antiserum to murine erythrocytes (anti-RBC) and compared it with rabbit antiserum generated ad hoc to murine L-cells (anti-L-cell). Our results indicate that anti-RBC is at least as effective as anti-L-cell serum for the immunosurgical isolation of inner cell masses, which became either miniblastocysts (later forming outgrowths) or embryoid bodies (undergoing ectoderm-endodermlike differentiation within 48 h). Because anti-RBC is commercially available, the technical modification described herein increases the accessibility of the immunosurgical protocol for the isolation of murine inner cell masses.en
dc.language.isoen_USen
dc.publisherSociety for In-Vitro Biology / Springer Verlagen
dc.identifier.doi10.1007/BF02634557en
dc.relation.urlhttp://link.springer.com/10.1007/BF02634557en
dc.subject.departmentBiologyen_US
dc.titleMouse blastocyst immunosurgery with commercial antiserum to mouse erythrocytesen_US
dc.typeArticleen
dc.identifier.journalIn Vitro Cellular and Developmental Biology - Animalen
dc.subject.keywordAnitbody cytotoxicityen_US
dc.subject.keywordImmunosurgeryen_US
dc.subject.keywordInner cell mass isolationen_US
dc.identifier.volume29en_US
dc.identifier.issue8en_US
dc.identifier.startpage671en_US
dc.rightsArchived with thanks to In Vitro Cellular & Developmental Biology - Animalen
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