A staging scheme for assessing development in vitro of organogenesis stage embryos of the stripe-faced dunnart, Sminthopsis macroura (Marsupialia: dasyuridae)

Title:
A staging scheme for assessing development in vitro of organogenesis stage embryos of the stripe-faced dunnart, Sminthopsis macroura (Marsupialia: dasyuridae)
Authors:
Cruz, Yolanda P.; Hickford, D.; Selwood, Lynne
Abstract:
The inaccessibility of mammalian organogenesis stage embryos has precluded their widespread use in embryological and teratological studies. As organogenesis occurs during the last 1.5 days of the 10. 7 days of gestation in the stripe-faced dunnart (Sminthopsis macroura), the aim of the present study was to investigate whether day 9 and day 10 embryos and fetuses could be grown to term in vitro. High glucose Dulbecco's modified Eagle's medium with 10% fetal calf serum (FCS) supported embryonic growth for various periods of time, some to within 5 h of the predicted time of parturition. A roller culture system maintained at 35 degrees C was used to incubate organogenesis stage embryos (n = 43). Nine unincubated (control) embryos were either fixed for microscopic analysis or frozen for microprotein determination. The results of the present study indicate that with some optimization of the culture conditions (increasing oxygen in the gas phase in the culture tubes, replacing FCS with rat serum), it might be possible for organogenesis stage S. macroura embryos to be grown to term. A scoring scheme for assessing morphological development was devised for use as a standard in staging organogenesis stage embryos. This scheme reflects the highly compressed schedule of developmental events that occurs mainly during day 9 of gestation in S. macroura embryos. In comparison, during embryogenesis in Didelphis virginiana these developmental events occur from day 8 to day 10.5 of gestation, and birth occurs on day 13.
Citation:
Cruz, Y.P., D. Hickford, and L. Selwood. 2000. "A staging scheme for assessing development in vitro of organogenesis stage embryos of the stripe-faced dunnart, Sminthopsis macroura (Marsupialia: dasyuridae)." Reproduction 120(1): 99-108.
Publisher:
BioScientifica / Society for Reproduction and Fertility
DATE ISSUED:
2000-09-01
Department:
Biology
Type:
Article
PUBLISHED VERSION:
10.1530/jrf.0.1200099
Additional Links:
http://www.reproduction-online.org/cgi/doi/10.1530/jrf.0.1200099
PERMANENT LINK:
http://hdl.handle.net/11282/600472

Full metadata record

DC FieldValue Language
dc.contributor.authorCruz, Yolanda P.en
dc.contributor.authorHickford, D.en
dc.contributor.authorSelwood, Lynneen
dc.date.accessioned2016-03-02T14:12:18Zen
dc.date.available2016-03-02T14:12:18Zen
dc.date.issued2000-09-01en
dc.identifier.citationCruz, Y.P., D. Hickford, and L. Selwood. 2000. "A staging scheme for assessing development in vitro of organogenesis stage embryos of the stripe-faced dunnart, Sminthopsis macroura (Marsupialia: dasyuridae)." Reproduction 120(1): 99-108.en
dc.identifier.issn1470-1626en
dc.identifier.urihttp://hdl.handle.net/11282/600472en
dc.description.abstractThe inaccessibility of mammalian organogenesis stage embryos has precluded their widespread use in embryological and teratological studies. As organogenesis occurs during the last 1.5 days of the 10. 7 days of gestation in the stripe-faced dunnart (Sminthopsis macroura), the aim of the present study was to investigate whether day 9 and day 10 embryos and fetuses could be grown to term in vitro. High glucose Dulbecco's modified Eagle's medium with 10% fetal calf serum (FCS) supported embryonic growth for various periods of time, some to within 5 h of the predicted time of parturition. A roller culture system maintained at 35 degrees C was used to incubate organogenesis stage embryos (n = 43). Nine unincubated (control) embryos were either fixed for microscopic analysis or frozen for microprotein determination. The results of the present study indicate that with some optimization of the culture conditions (increasing oxygen in the gas phase in the culture tubes, replacing FCS with rat serum), it might be possible for organogenesis stage S. macroura embryos to be grown to term. A scoring scheme for assessing morphological development was devised for use as a standard in staging organogenesis stage embryos. This scheme reflects the highly compressed schedule of developmental events that occurs mainly during day 9 of gestation in S. macroura embryos. In comparison, during embryogenesis in Didelphis virginiana these developmental events occur from day 8 to day 10.5 of gestation, and birth occurs on day 13.en
dc.language.isoen_USen
dc.publisherBioScientifica / Society for Reproduction and Fertilityen
dc.identifier.doi10.1530/jrf.0.1200099en
dc.relation.urlhttp://www.reproduction-online.org/cgi/doi/10.1530/jrf.0.1200099en
dc.subject.departmentBiologyen_US
dc.titleA staging scheme for assessing development in vitro of organogenesis stage embryos of the stripe-faced dunnart, Sminthopsis macroura (Marsupialia: dasyuridae)en_US
dc.typeArticleen
dc.identifier.journalReproductionen
dc.identifier.volume120en_US
dc.identifier.issue1en_US
dc.identifier.startpage99en_US
dc.rightsArchived with thanks to Reproductionen
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