Sequence and expression of the kettin gene in Drosophila melanogaster and Caenorhabditis elegans

Title:
Sequence and expression of the kettin gene in Drosophila melanogaster and Caenorhabditis elegans
Authors:
Kolmerer, Bernhard; Clayton, Jonathan; Benes, Vladimir; Allen, Taylor; Ferguson, Charles; Leonard, Kevin; Weber, Ursula; Knekt, Marika; Ansorge, Wilhelm; Labeit, Siegfried; Bullard, Belinda
Abstract:
Kettin is a large modular protein associated with thin filaments in the Z-disc region of insect muscles. The sequence of a 21.3 kb contig of the Drosophila gene has been determined. The corresponding protein sequence has 35 immunoglobulin-like (Ig) domains which are separated by shorter linker sequences, except near the N and C termini of the molecule where linker sequences are short or missing. This confirms a model in which each Ig domain binds to an actin protomer. The Drosophila kettin gene is at 62C 1-3 on the third chromosome. Two P-element insertions, l(3)j1D7 and l(3)rL182 are in the kettin gene, and complementation tests showed that existing l(3)dre8 mutations are in the same gene. The RNA was detected in wild-type Drosophila embryos at stage 11, first in the gut invagination region of the mesoderm, and by stage 13 in both visceral and somatic mesoderm. Somatic mesoderm expression became segmental at stage 13. RNA expression was greatly reduced in embryos of P-element homozygotes but normal in heterozygotes. The structure of the flight muscle in all the heterozygous mutants was normal, including the myofibril-cuticle connections, and they were able to fly. Kettin sequence homologous to the Drosophila protein, was identified in the Caenorhabditis elegans genome database. The RNA was detected in pharyngeal, body wall and anal depressor muscles of larvae and adult worms, as well as in the male gonad. Antibody to insect kettin labelled the pharyngeal, body wall, anal depressor and proximal gonadal muscles in adult worms. Body wall muscles were labelled in an obliquely striated pattern consistent with the Z-disc localisation in insect muscle. The relationship of kettin to D-titin, which has been assigned to the same chromosomal locus in Drosophila, is discussed.
Citation:
Kolmerer, B., J. Clayton, V. Benes, T. Allen, et al. 2000. "Sequence and expression of the kettin gene in Drosophila melanogaster and Caenorhabditis elegans." Journal of Molecular Biology 296(2): 435-448.
Publisher:
Elsevier for Academic Press
DATE ISSUED:
2000-02
Department:
Biology
Type:
Article
PUBLISHED VERSION:
10.1006/jmbi.1999.3461
Additional Links:
http://linkinghub.elsevier.com/retrieve/pii/S0022283699934617
PERMANENT LINK:
http://hdl.handle.net/11282/597054

Full metadata record

DC FieldValue Language
dc.contributor.authorKolmerer, Bernharden
dc.contributor.authorClayton, Jonathanen
dc.contributor.authorBenes, Vladimiren
dc.contributor.authorAllen, Tayloren
dc.contributor.authorFerguson, Charlesen
dc.contributor.authorLeonard, Kevinen
dc.contributor.authorWeber, Ursulaen
dc.contributor.authorKnekt, Marikaen
dc.contributor.authorAnsorge, Wilhelmen
dc.contributor.authorLabeit, Siegfrieden
dc.contributor.authorBullard, Belindaen
dc.date.accessioned2016-02-23T15:56:06Zen
dc.date.available2016-02-23T15:56:06Zen
dc.date.issued2000-02en
dc.identifier.citationKolmerer, B., J. Clayton, V. Benes, T. Allen, et al. 2000. "Sequence and expression of the kettin gene in Drosophila melanogaster and Caenorhabditis elegans." Journal of Molecular Biology 296(2): 435-448.en
dc.identifier.issn0022-2836en
dc.identifier.urihttp://hdl.handle.net/11282/597054en
dc.description.abstractKettin is a large modular protein associated with thin filaments in the Z-disc region of insect muscles. The sequence of a 21.3 kb contig of the Drosophila gene has been determined. The corresponding protein sequence has 35 immunoglobulin-like (Ig) domains which are separated by shorter linker sequences, except near the N and C termini of the molecule where linker sequences are short or missing. This confirms a model in which each Ig domain binds to an actin protomer. The Drosophila kettin gene is at 62C 1-3 on the third chromosome. Two P-element insertions, l(3)j1D7 and l(3)rL182 are in the kettin gene, and complementation tests showed that existing l(3)dre8 mutations are in the same gene. The RNA was detected in wild-type Drosophila embryos at stage 11, first in the gut invagination region of the mesoderm, and by stage 13 in both visceral and somatic mesoderm. Somatic mesoderm expression became segmental at stage 13. RNA expression was greatly reduced in embryos of P-element homozygotes but normal in heterozygotes. The structure of the flight muscle in all the heterozygous mutants was normal, including the myofibril-cuticle connections, and they were able to fly. Kettin sequence homologous to the Drosophila protein, was identified in the Caenorhabditis elegans genome database. The RNA was detected in pharyngeal, body wall and anal depressor muscles of larvae and adult worms, as well as in the male gonad. Antibody to insect kettin labelled the pharyngeal, body wall, anal depressor and proximal gonadal muscles in adult worms. Body wall muscles were labelled in an obliquely striated pattern consistent with the Z-disc localisation in insect muscle. The relationship of kettin to D-titin, which has been assigned to the same chromosomal locus in Drosophila, is discussed.en
dc.language.isoen_USen
dc.publisherElsevier for Academic Pressen
dc.identifier.doi10.1006/jmbi.1999.3461en
dc.relation.urlhttp://linkinghub.elsevier.com/retrieve/pii/S0022283699934617en
dc.subject.departmentBiologyen_US
dc.titleSequence and expression of the kettin gene in Drosophila melanogaster and Caenorhabditis elegansen_US
dc.typeArticleen
dc.identifier.journalJournal of Molecular Biologyen
dc.subject.keywordKettinen_US
dc.subject.keywordDrosophilaen_US
dc.subject.keywordCaenorhabditisen_US
dc.subject.keywordInsect muscleen_US
dc.subject.keywordZ-discen_US
dc.identifier.volume296en_US
dc.identifier.issue2en_US
dc.identifier.startpage435en_US
dc.rightsArchived with thanks to Journal of Molecular Biologyen
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