Calcium signaling and neuropeptide secretion in the expulsion step of the Caenorhabditis elegans defecation motor program

Title:
Calcium signaling and neuropeptide secretion in the expulsion step of the Caenorhabditis elegans defecation motor program
Authors:
McCright, Sam; Peters, Maureen A.
Abstract:
A periodic posterior to anterior intestinal calcium wave initiates the defecation motor program of Caenorhabditis elegans (C. elegans). innexin-16 (inx-16) encodes an intestinal pannexin gap junction subunit. inx-16 mutants have faulty calcium waves and mostly lack the expulsion step of the motor program. Our work investigates how these defective calcium waves result in loss of expulsion. The anterior-ventral neuron L (AVL) and dorsal-ventral neuron B (DVB) are responsible for expulsion through a two-step signaling process. An intestinal neuropeptide-like protein, NLP-40, activates AVL and DVB which in turn release neurotransmitters to stimulate expulsion. NLP-40 release likely depends on intestinal calcium flux, as vesicular localization of NLP-40 requires synaptotagmin-2. We propose that the expulsion defects in the inx-16 mutant are due to improper NLP-40 release. To establish if inx-16’s AVL and DVB neurons are functional, we used optogenetic methods. Activation of a light-gated ion channel channelrhodopsin-2 (ChR2) in AVL and DVB of inx-16 animals resulted in normal expulsion 90% of the time. This demonstrates that an inx-16 animal’s AVL and DVB neurons are able to signal expulsion and supports our hypothesis that defects in inx-16’s intestinal NLP-40 release inhibit expulsion. Current work addresses whether NLP-40 is properly synthesized and released.
Citation:
McCright, Sam, and Maureen Peters. 2014. "Calcium signaling and neuropeptide secretion in the expulsion step of the Caenorhabditis elegans defecation motor program." FASEB Journal 28(1): 1129.6
Publisher:
Federation of American Society of Experimental Biology (FASEB)
DATE ISSUED:
2014-04
Department:
Biology
Type:
Abstract
Additional Links:
http://www.fasebj.org/gca?allch=&submit=Go&gca=fasebj%3B28%2F1_Supplement%2F1129.6
Notes:
Supplement: S
PERMANENT LINK:
http://hdl.handle.net/11282/594823

Full metadata record

DC FieldValue Language
dc.contributor.authorMcCright, Samen
dc.contributor.authorPeters, Maureen A.en
dc.date.accessioned2016-01-25T19:50:37Zen
dc.date.available2016-01-25T19:50:37Zen
dc.date.issued2014-04en
dc.identifier.citationMcCright, Sam, and Maureen Peters. 2014. "Calcium signaling and neuropeptide secretion in the expulsion step of the Caenorhabditis elegans defecation motor program." FASEB Journal 28(1): 1129.6en
dc.identifier.issn0892-6638en
dc.identifier.urihttp://hdl.handle.net/11282/594823en
dc.description.abstractA periodic posterior to anterior intestinal calcium wave initiates the defecation motor program of Caenorhabditis elegans (C. elegans). innexin-16 (inx-16) encodes an intestinal pannexin gap junction subunit. inx-16 mutants have faulty calcium waves and mostly lack the expulsion step of the motor program. Our work investigates how these defective calcium waves result in loss of expulsion. The anterior-ventral neuron L (AVL) and dorsal-ventral neuron B (DVB) are responsible for expulsion through a two-step signaling process. An intestinal neuropeptide-like protein, NLP-40, activates AVL and DVB which in turn release neurotransmitters to stimulate expulsion. NLP-40 release likely depends on intestinal calcium flux, as vesicular localization of NLP-40 requires synaptotagmin-2. We propose that the expulsion defects in the inx-16 mutant are due to improper NLP-40 release. To establish if inx-16’s AVL and DVB neurons are functional, we used optogenetic methods. Activation of a light-gated ion channel channelrhodopsin-2 (ChR2) in AVL and DVB of inx-16 animals resulted in normal expulsion 90% of the time. This demonstrates that an inx-16 animal’s AVL and DVB neurons are able to signal expulsion and supports our hypothesis that defects in inx-16’s intestinal NLP-40 release inhibit expulsion. Current work addresses whether NLP-40 is properly synthesized and released.en_US
dc.language.isoen_USen
dc.publisherFederation of American Society of Experimental Biology (FASEB)en
dc.relation.urlhttp://www.fasebj.org/gca?allch=&submit=Go&gca=fasebj%3B28%2F1_Supplement%2F1129.6en
dc.subject.departmentBiologyen_US
dc.titleCalcium signaling and neuropeptide secretion in the expulsion step of the Caenorhabditis elegans defecation motor programen_US
dc.typeAbstracten
dc.description.notesSupplement: Sen_US
dc.identifier.journalFASEB Journalen
dc.identifier.volume28en_US
dc.identifier.issue1en_US
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