Ehs-1 is required for the expulsion step of the Caenorhabditis elegans defecation motor program

Title:
Ehs-1 is required for the expulsion step of the Caenorhabditis elegans defecation motor program
Authors:
Freeburg, Nelson; Peters, Maureen A.
Abstract:
The Caenorhabditis elegans digestive motor program occurs at approximately 50 second intervals and consists of three contractions. The first contraction occurs in the posterior body wall muscle and is followed by a contraction of the anterior body wall muscle approximately 4 seconds later. Finally, waste is expelled via contraction of the enteric muscles. The later contractions are controlled by the release of neuropeptides from the intestine, which activate two GABAergic neurons. A genome-wide RNA interference (RNAi) screen identified EPS-15 homologous sequence 1 (ehs-1) as a candidate gene involved in defecation. Ehs-1 knockdown does not affect the first contraction or the timekeeping mechanisms. However, the expulsion step occurs in only 10.9% percent of cycles. Ehs-1 has previously been implicated in synaptic vesicle recycling in neurons, suggesting that the expulsion defect is due to defective GABA release from DVB. However, intestine-specific knockdown of ehs-1 results in a 92.3% reduction in expulsion, matching the expulsion frequency following systemic knockdown. Our findings suggest that intestinal expression of ehs-1 is also required for expulsion. We hypothesize that ehs-1 is needed for recycling of vesicles in both neurons and intestines.
Citation:
Freeburg, Nelson, and Maureen Peters. 2014. "Ehs-1 is required for the expulsion step of the Caenorhabditis elegans defecation motor program." FASEB Journal 28(1): 1129.5.
Publisher:
Federation of American Society of Experimental Biology (FASEB)
DATE ISSUED:
2014-04
Department:
Biology
Type:
Abstract
Additional Links:
http://www.fasebj.org/gca?allch=&submit=Go&gca=fasebj%3B28%2F1_Supplement%2F1129.5
Notes:
Supplement: S
PERMANENT LINK:
http://hdl.handle.net/11282/594822

Full metadata record

DC FieldValue Language
dc.contributor.authorFreeburg, Nelsonen
dc.contributor.authorPeters, Maureen A.en
dc.date.accessioned2016-01-25T19:45:44Zen
dc.date.available2016-01-25T19:45:44Zen
dc.date.issued2014-04en
dc.identifier.citationFreeburg, Nelson, and Maureen Peters. 2014. "Ehs-1 is required for the expulsion step of the Caenorhabditis elegans defecation motor program." FASEB Journal 28(1): 1129.5.en
dc.identifier.issn0892-6638en
dc.identifier.urihttp://hdl.handle.net/11282/594822en
dc.description.abstractThe Caenorhabditis elegans digestive motor program occurs at approximately 50 second intervals and consists of three contractions. The first contraction occurs in the posterior body wall muscle and is followed by a contraction of the anterior body wall muscle approximately 4 seconds later. Finally, waste is expelled via contraction of the enteric muscles. The later contractions are controlled by the release of neuropeptides from the intestine, which activate two GABAergic neurons. A genome-wide RNA interference (RNAi) screen identified EPS-15 homologous sequence 1 (ehs-1) as a candidate gene involved in defecation. Ehs-1 knockdown does not affect the first contraction or the timekeeping mechanisms. However, the expulsion step occurs in only 10.9% percent of cycles. Ehs-1 has previously been implicated in synaptic vesicle recycling in neurons, suggesting that the expulsion defect is due to defective GABA release from DVB. However, intestine-specific knockdown of ehs-1 results in a 92.3% reduction in expulsion, matching the expulsion frequency following systemic knockdown. Our findings suggest that intestinal expression of ehs-1 is also required for expulsion. We hypothesize that ehs-1 is needed for recycling of vesicles in both neurons and intestines.en_US
dc.language.isoen_USen
dc.publisherFederation of American Society of Experimental Biology (FASEB)en
dc.relation.urlhttp://www.fasebj.org/gca?allch=&submit=Go&gca=fasebj%3B28%2F1_Supplement%2F1129.5en_US
dc.subject.departmentBiologyen_US
dc.titleEhs-1 is required for the expulsion step of the Caenorhabditis elegans defecation motor programen_US
dc.typeAbstracten
dc.description.notesSupplement: Sen_US
dc.identifier.journalFASEB Journalen
dc.identifier.volume28en_US
dc.identifier.issue1en_US
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