Lineage-specific gene radiations underlie the evolution of novel betalain pigmentation in Caryophyllales

Title:
Lineage-specific gene radiations underlie the evolution of novel betalain pigmentation in Caryophyllales
Authors:
Brockington, Samuel F.; Yang, Ya; Gandia-Herrero, Fernando; Covshoff, Sarah; Hibberd, Julian M.; Sage, Rowan F.; Wong, Gane Ka-Shu; Moore, Michael J.; Smith, Stephen A.
Abstract:
Betalain pigments are unique to the Caryophyllales and structurally and biosynthetically distinct from anthocyanins. Two key enzymes within the betalain synthesis pathway have been identified: 4,5-dioxygenase (DODA) that catalyzes the formation of betalamic acid and CYP76AD1, a cytochrome P450 gene that catalyzes the formation of cyclo-DOPA. We performed phylogenetic analyses to reveal the evolutionary history of the DODA and CYP76AD1 lineages and in the context of an ancestral reconstruction of pigment states we explored the evolution of these genes in relation to the complex evolution of pigments in Caryophylalles. Duplications within the CYP76AD1 and DODA lineages arose just before the origin of betalain pigmentation in the core Caryophyllales. The duplications gave rise to DODA- and CYP76AD1- isoforms that appear specific to betalain synthesis. Both betalain-specific isoforms were then lost or downregulated in the anthocyanic Molluginaceae and Caryophyllaceae. Our findings suggest a single origin of the betalain synthesis pathway, with neofunctionalization following gene duplications in the CYP76AD1 and DODA lineages. Loss of DODA- and CYP76AD1- in anthocyanic taxa suggests that betalain pigmentation has been lost twice in Caryophyllales, and exclusion of betalain pigments from anthocyanic taxa is mediated through gene loss or downregulation. [Correction added after online publication 13 May 2015: in the last two paragraphs of the Summary the gene name CYP761A was changed to CYP76AD1.]
Citation:
Brockington, S.F., Y. Yang, F. Gandi-Herrero, et al. 2015. "Lineage-specific gene radiations underlie the evolution of novel betalain pigmentation in Caryophyllales." New Phytologist 207(4): 1170-1180.
Publisher:
Wiley for New Phytologist Trust
DATE ISSUED:
2015-09
Department:
Biology
Type:
Article
PUBLISHED VERSION:
10.1111/nph.13441
PERMANENT LINK:
http://hdl.handle.net/11282/594055

Full metadata record

DC FieldValue Language
dc.contributor.authorBrockington, Samuel F.en
dc.contributor.authorYang, Yaen
dc.contributor.authorGandia-Herrero, Fernandoen
dc.contributor.authorCovshoff, Sarahen
dc.contributor.authorHibberd, Julian M.en
dc.contributor.authorSage, Rowan F.en
dc.contributor.authorWong, Gane Ka-Shuen
dc.contributor.authorMoore, Michael J.en
dc.contributor.authorSmith, Stephen A.en
dc.date.accessioned2016-01-19T13:06:37Zen
dc.date.available2016-01-19T13:06:37Zen
dc.date.issued2015-09en
dc.identifier.citationBrockington, S.F., Y. Yang, F. Gandi-Herrero, et al. 2015. "Lineage-specific gene radiations underlie the evolution of novel betalain pigmentation in Caryophyllales." New Phytologist 207(4): 1170-1180.en
dc.identifier.issn0028-646Xen
dc.identifier.urihttp://hdl.handle.net/11282/594055en
dc.description.abstractBetalain pigments are unique to the Caryophyllales and structurally and biosynthetically distinct from anthocyanins. Two key enzymes within the betalain synthesis pathway have been identified: 4,5-dioxygenase (DODA) that catalyzes the formation of betalamic acid and CYP76AD1, a cytochrome P450 gene that catalyzes the formation of cyclo-DOPA. We performed phylogenetic analyses to reveal the evolutionary history of the DODA and CYP76AD1 lineages and in the context of an ancestral reconstruction of pigment states we explored the evolution of these genes in relation to the complex evolution of pigments in Caryophylalles. Duplications within the CYP76AD1 and DODA lineages arose just before the origin of betalain pigmentation in the core Caryophyllales. The duplications gave rise to DODA- and CYP76AD1- isoforms that appear specific to betalain synthesis. Both betalain-specific isoforms were then lost or downregulated in the anthocyanic Molluginaceae and Caryophyllaceae. Our findings suggest a single origin of the betalain synthesis pathway, with neofunctionalization following gene duplications in the CYP76AD1 and DODA lineages. Loss of DODA- and CYP76AD1- in anthocyanic taxa suggests that betalain pigmentation has been lost twice in Caryophyllales, and exclusion of betalain pigments from anthocyanic taxa is mediated through gene loss or downregulation. [Correction added after online publication 13 May 2015: in the last two paragraphs of the Summary the gene name CYP761A was changed to CYP76AD1.]en
dc.language.isoen_USen
dc.publisherWiley for New Phytologist Trusten
dc.identifier.doi10.1111/nph.13441en
dc.subject.departmentBiologyen_US
dc.titleLineage-specific gene radiations underlie the evolution of novel betalain pigmentation in Caryophyllalesen_US
dc.typeArticleen
dc.identifier.journalNew Phytologisten
dc.subject.keywordAnthocyaninen_US
dc.subject.keywordBetalainsen_US
dc.subject.keywordCaryophyllalesen_US
dc.subject.keywordLineage-specific genesen_US
dc.subject.keywordPigmentationen_US
dc.subject.keywordTaxonomically restricted genesen_US
dc.identifier.volume207en_US
dc.identifier.issue4en_US
dc.identifier.startpage1170en_US
dc.rightsArchived with thanks to New Phytologisten
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