The architecture of a scrambled genome reveals massive levels of genomic rearrangement during development

Title:
The architecture of a scrambled genome reveals massive levels of genomic rearrangement during development
Authors:
Chen, Xiao; Bracht, John R.; Goldman, Aaron David; Dolzhenko, Egor; Clay, Derek M.; Swart, Estienne C.; Perlman, David H.; Doak, Thomas G.; Stuart, Andrew; Amemiya, Chris T.; Sebra, Robert P.; Landweber, Laura F.
Abstract:
Programmed DNA rearrangements in the single-celled eukaryote Oxytricha trifallax completely rewire its germline into a somatic nucleus during development. This elaborate, RNA-mediated pathway eliminates noncoding DNA sequences that interrupt gene loci and reorganizes the remaining fragments by inversions and permutations to produce functional genes. Here, we report the Oxytricha germline genome and compare it to the somatic genome to present a global view of its massive scale of genome rearrangements. The remarkably encrypted genome architecture contains >3,500 scrambled genes, as well as >800 predicted germline-limited genes expressed, and some posttranslationally modified, during genome rearrangements. Gene segments for different somatic loci often interweave with each other. Single gene segments can contribute to multiple, distinct somatic loci. Terminal precursor segments from neighboring somatic loci map extremely close to each other, often overlapping. This genome assembly provides a draft of a scrambled genome and a powerful model for studies of genome rearrangement.
Citation:
Chen X., J.R. Bracht, A.D. Goldman, E. Dolzhenko, D.M. Clay, E.C. Swart, D.H. Perlman, T.G. Doak, A. Stuart, C.T. Amemiya, R.P. Sebra, and L.F. Landweber. August 2014. "The architecture of a scrambled genome reveals massive levels of genomic rearrangement during development." Cell 158(5): 1187–1198.
Publisher:
Cell Press
DATE ISSUED:
2015-08-13
Department:
Biology
Type:
Article
PUBLISHED VERSION:
10.1016/j.cell.2014.07.034
Additional Links:
http://www.cell.com/cell/abstract/S0092-8674%2814%2900984-2
PERMANENT LINK:
http://hdl.handle.net/11282/566900

Full metadata record

DC FieldValue Language
dc.contributor.authorChen, Xiaoen
dc.contributor.authorBracht, John R.en
dc.contributor.authorGoldman, Aaron Daviden
dc.contributor.authorDolzhenko, Egoren
dc.contributor.authorClay, Derek M.en
dc.contributor.authorSwart, Estienne C.en
dc.contributor.authorPerlman, David H.en
dc.contributor.authorDoak, Thomas G.en
dc.contributor.authorStuart, Andrewen
dc.contributor.authorAmemiya, Chris T.en
dc.contributor.authorSebra, Robert P.en
dc.contributor.authorLandweber, Laura F.en
dc.date.accessioned2015-08-13T10:36:35Zen
dc.date.available2015-08-13T10:36:35Zen
dc.date.issued2015-08-13en
dc.identifier.citationChen X., J.R. Bracht, A.D. Goldman, E. Dolzhenko, D.M. Clay, E.C. Swart, D.H. Perlman, T.G. Doak, A. Stuart, C.T. Amemiya, R.P. Sebra, and L.F. Landweber. August 2014. "The architecture of a scrambled genome reveals massive levels of genomic rearrangement during development." Cell 158(5): 1187–1198.en
dc.identifier.issn0092-8674en
dc.identifier.urihttp://hdl.handle.net/11282/566900en
dc.description.abstractProgrammed DNA rearrangements in the single-celled eukaryote Oxytricha trifallax completely rewire its germline into a somatic nucleus during development. This elaborate, RNA-mediated pathway eliminates noncoding DNA sequences that interrupt gene loci and reorganizes the remaining fragments by inversions and permutations to produce functional genes. Here, we report the Oxytricha germline genome and compare it to the somatic genome to present a global view of its massive scale of genome rearrangements. The remarkably encrypted genome architecture contains >3,500 scrambled genes, as well as >800 predicted germline-limited genes expressed, and some posttranslationally modified, during genome rearrangements. Gene segments for different somatic loci often interweave with each other. Single gene segments can contribute to multiple, distinct somatic loci. Terminal precursor segments from neighboring somatic loci map extremely close to each other, often overlapping. This genome assembly provides a draft of a scrambled genome and a powerful model for studies of genome rearrangement.en
dc.language.isoen_USen
dc.publisherCell Pressen
dc.identifier.doi10.1016/j.cell.2014.07.034en
dc.relation.urlhttp://www.cell.com/cell/abstract/S0092-8674%2814%2900984-2en_US
dc.subject.departmentBiologyen
dc.titleThe architecture of a scrambled genome reveals massive levels of genomic rearrangement during developmenten
dc.typeArticleen
dc.identifier.journalCellen
dc.identifier.volume158en
dc.identifier.issue5en
dc.identifier.startpage1187en
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