Investigation of Model Cell Membranes with Raman Spectroscopy: A Biochemistry Laboratory Experiment

Title:
Investigation of Model Cell Membranes with Raman Spectroscopy: A Biochemistry Laboratory Experiment
Authors:
Craig, Norman C.; Fuchsman, William H.; Lacuesta, Nanette N.
Abstract:
New developments that extend the usefulness of Raman spectroscopy for the investigation of biological systems are described. One of these methods, excitation with a near-infrared laser at 1064 nm and detection with a Fourier transform infrared spectrometer, is applied to the investigation of temperature profiles of dispersions of phospholipids in water. These dispersions are models of biomembranes. This experiment for the undergraduate biochemisty laboratory gives students experience applying vibrational spectroscopy to the investigation of the molecular properties of biomembranes. The basis of the Raman effect is reviewed.
Citation:
Craig, Norman C., William H. Fuchsman, and Nanette N. Lacuesta. 2003. "Investigation of Model Cell Membranes with Raman Spectroscopy: A Biochemistry Laboratory Experiment." Journal of Chemical Education 80(11): 1282.
Publisher:
American Chemical Society, Division of Chemical Education
DATE ISSUED:
2003
Department:
Chemistry
Type:
Article
PUBLISHED VERSION:
10.1021/ed080p1282
PERMANENT LINK:
http://hdl.handle.net/11282/334046

Full metadata record

DC FieldValue Language
dc.contributor.authorCraig, Norman C.en
dc.contributor.authorFuchsman, William H.en
dc.contributor.authorLacuesta, Nanette N.en
dc.date.accessioned2014-11-10T14:13:01Z-
dc.date.available2014-11-10T14:13:01Z-
dc.date.issued2003en
dc.identifier.citationCraig, Norman C., William H. Fuchsman, and Nanette N. Lacuesta. 2003. "Investigation of Model Cell Membranes with Raman Spectroscopy: A Biochemistry Laboratory Experiment." Journal of Chemical Education 80(11): 1282.en
dc.identifier.issn0021-9584en
dc.identifier.urihttp://hdl.handle.net/11282/334046-
dc.description.abstractNew developments that extend the usefulness of Raman spectroscopy for the investigation of biological systems are described. One of these methods, excitation with a near-infrared laser at 1064 nm and detection with a Fourier transform infrared spectrometer, is applied to the investigation of temperature profiles of dispersions of phospholipids in water. These dispersions are models of biomembranes. This experiment for the undergraduate biochemisty laboratory gives students experience applying vibrational spectroscopy to the investigation of the molecular properties of biomembranes. The basis of the Raman effect is reviewed.en
dc.language.isoen_USen
dc.publisherAmerican Chemical Society, Division of Chemical Educationen
dc.identifier.doi10.1021/ed080p1282en
dc.subject.departmentChemistryen
dc.titleInvestigation of Model Cell Membranes with Raman Spectroscopy: A Biochemistry Laboratory Experimenten
dc.typeArticleen
dc.identifier.journalJournal of Chemical Educationen
dc.subject.keywordFourier transform techniquesen
dc.identifier.volume80en
dc.identifier.issue11en
dc.identifier.startpage1282en
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