Phyllotaxis and Rhizotaxis in Arabidopsis Are Modified by Three PLETHORA Transcription Factors

Title:
Phyllotaxis and Rhizotaxis in Arabidopsis Are Modified by Three PLETHORA Transcription Factors
Authors:
Hofhuis, Hugo; Laskowski, Marta ( 0000-0002-8875-7627 ) ; Du, Yujuan; Prasad, Kalika; Grigg, Stephen; Pinon, Violaine; Scheres, Ben
Abstract:
Background: The juxtaposition of newly formed primordia in the root and shoot differs greatly, but their formation in both contexts depends on local accumulation of the signaling molecule auxin. Whether the spacing of lateral roots along the main root and the arrangement of leaf primordia at the plant apex are controlled by related underlying mechanisms has remained unclear. Results: Here, we show that, in Arabidopsis thaliana, three transcriptional regulators implicated in phyllotaxis, PLETHORA3 (PLT3), PLT5, and PLT7, are expressed in incipient lateral root primordia where they are required for primordium development and lateral root emergence. Furthermore, all three PLT proteins prevent the formation of primordia close to one another, because, in their absence, successive lateral root primordia are frequently grouped in close longitudinal or radial clusters. The triple plt mutant phenotype is rescued by PLT-vYFP fusion proteins, which are expressed in the shoot meristem as well as the root, but not by expression of PLT7 in the shoot alone. Expression of all three PLT genes requires auxin response factors ARF7 and ARF19, and the reintroduction of PLT activity suffices to rescue lateral root formation in arf7,arf19. Conclusions: Intriguingly PLT 3, PLT5, and PLT7 not only control the positioning of organs at the shoot meristem but also in the root; a striking observation that raises many evolutionary questions.
Citation:
Hofhuis H., M. Laskowski, Y. Du, K. Prasad, S. Grigg, V. Pinon, and B. Scheres. June 2013. “Phyllotaxis and Rhizotaxis in Arabidopsis Are Modified by Three PLETHORA Transcription Factors.” Current Biology. 23(11): 956-62.  
Publisher:
Elsevier
DATE ISSUED:
2013
Department:
Biology
Type:
Article
PUBLISHED VERSION:
10.1016/j.cub.2013.04.048
Additional Links:
http://www.ncbi.nlm.nih.gov/pubmed/23684976
PERMANENT LINK:
http://hdl.handle.net/11282/332568

Full metadata record

DC FieldValue Language
dc.contributor.authorHofhuis, Hugoen
dc.contributor.authorLaskowski, Martaen
dc.contributor.authorDu, Yujuanen
dc.contributor.authorPrasad, Kalikaen
dc.contributor.authorGrigg, Stephenen
dc.contributor.authorPinon, Violaineen
dc.contributor.authorScheres, Benen
dc.date.accessioned2014-10-09T12:05:10Zen
dc.date.available2014-10-09T12:05:10Zen
dc.date.issued2013en
dc.identifier.citationHofhuis H., M. Laskowski, Y. Du, K. Prasad, S. Grigg, V. Pinon, and B. Scheres. June 2013. “Phyllotaxis and Rhizotaxis in Arabidopsis Are Modified by Three PLETHORA Transcription Factors.” Current Biology. 23(11): 956-62.  en
dc.identifier.issn0960-9822en
dc.identifier.urihttp://hdl.handle.net/11282/332568en
dc.description.abstractBackground: The juxtaposition of newly formed primordia in the root and shoot differs greatly, but their formation in both contexts depends on local accumulation of the signaling molecule auxin. Whether the spacing of lateral roots along the main root and the arrangement of leaf primordia at the plant apex are controlled by related underlying mechanisms has remained unclear. Results: Here, we show that, in Arabidopsis thaliana, three transcriptional regulators implicated in phyllotaxis, PLETHORA3 (PLT3), PLT5, and PLT7, are expressed in incipient lateral root primordia where they are required for primordium development and lateral root emergence. Furthermore, all three PLT proteins prevent the formation of primordia close to one another, because, in their absence, successive lateral root primordia are frequently grouped in close longitudinal or radial clusters. The triple plt mutant phenotype is rescued by PLT-vYFP fusion proteins, which are expressed in the shoot meristem as well as the root, but not by expression of PLT7 in the shoot alone. Expression of all three PLT genes requires auxin response factors ARF7 and ARF19, and the reintroduction of PLT activity suffices to rescue lateral root formation in arf7,arf19. Conclusions: Intriguingly PLT 3, PLT5, and PLT7 not only control the positioning of organs at the shoot meristem but also in the root; a striking observation that raises many evolutionary questions.en
dc.language.isoen_USen
dc.publisherElsevieren
dc.identifier.doi10.1016/j.cub.2013.04.048en
dc.relation.urlhttp://www.ncbi.nlm.nih.gov/pubmed/23684976en
dc.subject.departmentBiologyen
dc.titlePhyllotaxis and Rhizotaxis in Arabidopsis Are Modified by Three PLETHORA Transcription Factorsen
dc.typeArticleen
dc.identifier.journalCurrent Biologyen
dc.subject.keywordLateral root initiationen_US
dc.subject.keywordAuxin transporten_US
dc.subject.keywordThalianaen_US
dc.subject.keywordARF19en_US
dc.subject.keywordNPH4/ARF7en_US
dc.subject.keywordProteinsen_US
dc.identifier.volume23en
dc.identifier.issue11en
dc.identifier.startpage956en
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