Multivalent Interactions Between Lectins and Supramolecular Complexes: Galectin-1 and Self-Assembled Pseudopolyrotaxanes

Title:
Multivalent Interactions Between Lectins and Supramolecular Complexes: Galectin-1 and Self-Assembled Pseudopolyrotaxanes
Authors:
Belitsky, Jason M.; Nelson, Alshakim; Hernandez, Joseph D.; Baum, Linda G.; Stoddart, J. Fraser
Abstract:
Supramolecular chemistry has been employed to develop flexible and adaptable multivalent neoglycoconjugates for binding galectin-1 (Gal-1). Gal-1, a dimeric lectin with two galactoside-binding sites, regulates cancer progression and immune responses. Self-assembled pseudopolyrotaxanes consisting of lactoside-displaying cyclodextrin (LCD) “beads” threaded onto polyviologen “strings” display mobile ligands as a result of cyclodextrin rotation about, and limited translation along, the polymer chain. The pseudopolyrotaxanes rapidly and efficiently precipitate Gal-1 and provide valency-corrected enhancements of up to 30-fold compared to native lactose and 20-fold over free LCD in a T-cell agglutination assay. A supramolecular statistical effect was observed, wherein the efficacy of Gal-1 inhibition correlates with the number of ligands connected to each other solely through mechanical and noncovalent interactions. Such flexible and adaptable self-assembled pseudopolyrotaxanes show promise for the study of multivalent interactions and targeting of therapeutically relevant lectins.
Citation:
Belitsky, J.M., A. Nelson, J.D. Hernandez, L.G. Baum, et al. 2007. "Multivalent Interactions Between Lectins and Supramolecular Complexes: Galectin-1 and Self-Assembled Pseudopolyrotaxanes." Chemistry & Biology 14(10): 1140-1151.
Publisher:
Elsevier (Cell Press)
DATE ISSUED:
2007
Department:
Chemistry
Type:
article
PUBLISHED VERSION:
10.1016/j.chembiol.2007.09.007
Additional Links:
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2072908/
PERMANENT LINK:
http://hdl.handle.net/11282/310437

Full metadata record

DC FieldValue Language
dc.contributor.authorBelitsky, Jason M.en_US
dc.contributor.authorNelson, Alshakimen_US
dc.contributor.authorHernandez, Joseph D.en_US
dc.contributor.authorBaum, Linda G.en_US
dc.contributor.authorStoddart, J. Fraseren_US
dc.date.accessioned2013-12-23T16:33:08Zen
dc.date.available2013-12-23T16:33:08Zen
dc.date.issued2007en
dc.identifier.citationBelitsky, J.M., A. Nelson, J.D. Hernandez, L.G. Baum, et al. 2007. "Multivalent Interactions Between Lectins and Supramolecular Complexes: Galectin-1 and Self-Assembled Pseudopolyrotaxanes." Chemistry & Biology 14(10): 1140-1151.en_US
dc.identifier.issn1074-5521en_US
dc.identifier.urihttp://hdl.handle.net/11282/310437en
dc.description.abstractSupramolecular chemistry has been employed to develop flexible and adaptable multivalent neoglycoconjugates for binding galectin-1 (Gal-1). Gal-1, a dimeric lectin with two galactoside-binding sites, regulates cancer progression and immune responses. Self-assembled pseudopolyrotaxanes consisting of lactoside-displaying cyclodextrin (LCD) “beads” threaded onto polyviologen “strings” display mobile ligands as a result of cyclodextrin rotation about, and limited translation along, the polymer chain. The pseudopolyrotaxanes rapidly and efficiently precipitate Gal-1 and provide valency-corrected enhancements of up to 30-fold compared to native lactose and 20-fold over free LCD in a T-cell agglutination assay. A supramolecular statistical effect was observed, wherein the efficacy of Gal-1 inhibition correlates with the number of ligands connected to each other solely through mechanical and noncovalent interactions. Such flexible and adaptable self-assembled pseudopolyrotaxanes show promise for the study of multivalent interactions and targeting of therapeutically relevant lectins.en_US
dc.publisherElsevier (Cell Press)en_US
dc.identifier.doi10.1016/j.chembiol.2007.09.007en
dc.relation.urlhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC2072908/en_US
dc.subject.departmentChemistryen_US
dc.titleMultivalent Interactions Between Lectins and Supramolecular Complexes: Galectin-1 and Self-Assembled Pseudopolyrotaxanesen_US
dc.typearticleen_US
dc.identifier.journalChemistry & Biologyen_US
dc.identifier.volume14en_US
dc.identifier.issue10en_US
dc.identifier.startpage1140en_US
All Items in The Five Colleges of Ohio Digital Repository are protected by copyright, with all rights reserved, unless otherwise indicated.