Targeting Galectin-1 with Self-Assembled Multivalent Pseudopolyrotaxanes

Title:
Targeting Galectin-1 with Self-Assembled Multivalent Pseudopolyrotaxanes
Authors:
Belitsky, Jason M.; Stoddart, J. Fraser
Abstract:
This review describes the development of self-assembled multivalent pseudopolyrotaxanes as flexible and dynamic neoglycoconjugates for binding Galectin-1 (Gal-1). Gal-1, a dimeric lectin with two lactoside-binding sites, plays multiple roles in a variety of cancers. Pseudopolyrotaxanes comprised of lactoside-displaying cyclodextrin (LCD) "beads" threaded onto polyviologen "strings" display highly flexible and adaptable ligands as a result of rotation of the cyclodextrin torus about, and limited translation along, the polymer chain. The pseudopolyrotaxanes rapidly and efficiently precipitate Gal-1 and provide valency-corrected enhancements of up to 30-fold over native lactose and 20-fold over free LCD in a T-cell agglutination assay. These results show that the flexible and dynamic ligand presentation afforded by supramolecular assemblies, such as the pseudopolyrotaxanes, is a useful strategy for the study of protein-carbohydrate interactions and the exploitation of multivalency for targeting therapeutically relevant lectins.
Citation:
Belitsky, J.M., and J.F. Stoddart. "Targeting Galectin-1 with Self-Assembled Multivalent Pseudopolyrotaxanes." In Frontiers in Carbohydrate Chemistry, ACS Symposium series, edited by Alexei V. Demchenko. New York: Oxford University Press, 2007.
DATE ISSUED:
2007
Department:
Chemistry
Type:
Book, Section
PUBLISHED VERSION:
10.1021/bk-2007-0960.ch019
PERMANENT LINK:
http://hdl.handle.net/11282/310366

Full metadata record

DC FieldValue Language
dc.contributor.authorBelitsky, Jason M.en_US
dc.contributor.authorStoddart, J. Fraseren_US
dc.date.accessioned2013-12-23T16:31:33Z-
dc.date.available2013-12-23T16:31:33Z-
dc.date.issued2007en
dc.identifier.citationBelitsky, J.M., and J.F. Stoddart. "Targeting Galectin-1 with Self-Assembled Multivalent Pseudopolyrotaxanes." In Frontiers in Carbohydrate Chemistry, ACS Symposium series, edited by Alexei V. Demchenko. New York: Oxford University Press, 2007.en_US
dc.identifier.urihttp://hdl.handle.net/11282/310366-
dc.description.abstractThis review describes the development of self-assembled multivalent pseudopolyrotaxanes as flexible and dynamic neoglycoconjugates for binding Galectin-1 (Gal-1). Gal-1, a dimeric lectin with two lactoside-binding sites, plays multiple roles in a variety of cancers. Pseudopolyrotaxanes comprised of lactoside-displaying cyclodextrin (LCD) "beads" threaded onto polyviologen "strings" display highly flexible and adaptable ligands as a result of rotation of the cyclodextrin torus about, and limited translation along, the polymer chain. The pseudopolyrotaxanes rapidly and efficiently precipitate Gal-1 and provide valency-corrected enhancements of up to 30-fold over native lactose and 20-fold over free LCD in a T-cell agglutination assay. These results show that the flexible and dynamic ligand presentation afforded by supramolecular assemblies, such as the pseudopolyrotaxanes, is a useful strategy for the study of protein-carbohydrate interactions and the exploitation of multivalency for targeting therapeutically relevant lectins.en_US
dc.identifier.doi10.1021/bk-2007-0960.ch019-
dc.subject.departmentChemistryen_US
dc.titleTargeting Galectin-1 with Self-Assembled Multivalent Pseudopolyrotaxanesen_US
dc.typeBook, Sectionen_US
dc.identifier.volume960en_US
dc.identifier.startpage356en_US
All Items in The Five Colleges of Ohio Digital Repository are protected by copyright, with all rights reserved, unless otherwise indicated.