Determination of Global Structure from Distance and Orientation Constraints in Biological Solids Using Solid-State NMR Spectroscopy

Title:
Determination of Global Structure from Distance and Orientation Constraints in Biological Solids Using Solid-State NMR Spectroscopy
Authors:
Andreas, Loren B.; Mehta, Anil K.
Abstract:
We report the results from a new solid-state NMR experiment, DANTE−REDOR, which can determine global secondary structure in uniformly (13C,15N)-enriched systems by simultaneously measuring distance and orientation constraints. Following a heteronuclear spin-pair selection using a DANTE pulse train, the magnitude and orientation of the internuclear dipole vector, within the chemical shift anisotropy (CSA) frame of the observed nucleus, are determined by tracking the dephasing of individual spinning sidebands under magic angle spinning. The efficacy of the experiment is demonstrated by measuring the imidazole side-chain orientation in U-[13C6,15N3]-l-histidine·HCl·H2O.
Citation:
Andreas, Loren B., Loren B. Andreas, and Anil K. Mehta. 2007. "Determination of Global Structure from Distance and Orientation Constraints in Biological Solids Using Solid-State NMR Spectroscopy." Journal Of The American Chemical Society 129(49): 15233-15239.
Publisher:
American Chemical Society
DATE ISSUED:
2007
Department:
Chemistry
Type:
article
PUBLISHED VERSION:
10.1021/ja074789q
PERMANENT LINK:
http://hdl.handle.net/11282/310364

Full metadata record

DC FieldValue Language
dc.contributor.authorAndreas, Loren B.en_US
dc.contributor.authorMehta, Anil K.en_US
dc.date.accessioned2013-12-23T16:31:31Z-
dc.date.available2013-12-23T16:31:31Z-
dc.date.issued2007en
dc.identifier.citationAndreas, Loren B., Loren B. Andreas, and Anil K. Mehta. 2007. "Determination of Global Structure from Distance and Orientation Constraints in Biological Solids Using Solid-State NMR Spectroscopy." Journal Of The American Chemical Society 129(49): 15233-15239.en_US
dc.identifier.issn0002-7863en_US
dc.identifier.urihttp://hdl.handle.net/11282/310364-
dc.description.abstractWe report the results from a new solid-state NMR experiment, DANTE−REDOR, which can determine global secondary structure in uniformly (13C,15N)-enriched systems by simultaneously measuring distance and orientation constraints. Following a heteronuclear spin-pair selection using a DANTE pulse train, the magnitude and orientation of the internuclear dipole vector, within the chemical shift anisotropy (CSA) frame of the observed nucleus, are determined by tracking the dephasing of individual spinning sidebands under magic angle spinning. The efficacy of the experiment is demonstrated by measuring the imidazole side-chain orientation in U-[13C6,15N3]-l-histidine·HCl·H2O.en_US
dc.publisherAmerican Chemical Societyen_US
dc.identifier.doi10.1021/ja074789q-
dc.subject.departmentChemistryen_US
dc.titleDetermination of Global Structure from Distance and Orientation Constraints in Biological Solids Using Solid-State NMR Spectroscopyen_US
dc.typearticleen_US
dc.identifier.journalJournal Of The American Chemical Societyen_US
dc.identifier.volume129en_US
dc.identifier.issue49en_US
dc.identifier.startpage15233en_US
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