Expression Pattern Of E-cad, Ocln And Zo-1 In Cleavage-stage Monodelphis Domestica Embryos

Title:
Expression Pattern Of E-cad, Ocln And Zo-1 In Cleavage-stage Monodelphis Domestica Embryos
Authors:
Wang, Vicki N.; Cruz, Yolanda P.
Abstract:
During blastocyst formation in the laboratory opossum, M. domestica, each blastomere adheres to the zona pellucida, flattens against the zona, and later forms tight junctions with its neighbors. This contrasts greatly with mouse blastocyst formation, wherein cells adhere to each other and later form tight junctions, at no time adhering to the zona. We hypothesized that the spatio-temporal expression of the proteins encoded by E-Cad, Ocln and ZO-1 may explain the difference between eutherian (mouse) and marsupial (opossum) blastocyst formation. Confocal microscopy revealed that E-cadherin is found in both cytoplasm and embryonic coats (zona pellucida, mucoid layer). Its presence in the cytoplasm declined as blastocyst formation became imminent. The tight-junction proteins occludin and zona occludens were expressed similarly in the cytoplasm, although occludin later appeared in the perivitelline space, eventually becoming contained in the blastocoel. Both occludin and zona occludens were detected in zygotic nuclei. Occludin reappeared in larger amounts in cell nuclei during blastocyst formation. The presence of these cell-membrane proteins in nuclei warrants further investigation. Although the antibodies used in this investigation were molecularly similar only E-cadherin, not occludin or zona occludens, was detected in extracellular coats. It is thus unlikely that this E-cadherin expression pattern is artifactual. Rather, its presence in embryonic coats as well as cell surfaces (as occurs in mouse) suggests that it may facilitate blastomere-zona adhesion well in advance of blastomere-blastomere adhesion.
Citation:
Wang, Vicki N., and Yolanda P. Cruz. 2010. "Expression Pattern Of E-cad, Ocln And Zo-1 In Cleavage-stage Monodelphis Domestica Embryos." Developmental Biology 344(1): 75.
Publisher:
Academic Press/Elsevier Science
DATE ISSUED:
2010-08
Department:
Biology
Type:
article
PUBLISHED VERSION:
10.1016/j.ydbio.2010.05.114
PERMANENT LINK:
http://hdl.handle.net/11282/309938

Full metadata record

DC FieldValue Language
dc.contributor.authorWang, Vicki N.en_US
dc.contributor.authorCruz, Yolanda P.en_US
dc.date.accessioned2013-12-23T16:21:36Zen
dc.date.available2013-12-23T16:21:36Zen
dc.date.issued2010-08en
dc.identifier.citationWang, Vicki N., and Yolanda P. Cruz. 2010. "Expression Pattern Of E-cad, Ocln And Zo-1 In Cleavage-stage Monodelphis Domestica Embryos." Developmental Biology 344(1): 75.en_US
dc.identifier.issn0012-1606en_US
dc.identifier.urihttp://hdl.handle.net/11282/309938en
dc.description.abstractDuring blastocyst formation in the laboratory opossum, M. domestica, each blastomere adheres to the zona pellucida, flattens against the zona, and later forms tight junctions with its neighbors. This contrasts greatly with mouse blastocyst formation, wherein cells adhere to each other and later form tight junctions, at no time adhering to the zona. We hypothesized that the spatio-temporal expression of the proteins encoded by E-Cad, Ocln and ZO-1 may explain the difference between eutherian (mouse) and marsupial (opossum) blastocyst formation. Confocal microscopy revealed that E-cadherin is found in both cytoplasm and embryonic coats (zona pellucida, mucoid layer). Its presence in the cytoplasm declined as blastocyst formation became imminent. The tight-junction proteins occludin and zona occludens were expressed similarly in the cytoplasm, although occludin later appeared in the perivitelline space, eventually becoming contained in the blastocoel. Both occludin and zona occludens were detected in zygotic nuclei. Occludin reappeared in larger amounts in cell nuclei during blastocyst formation. The presence of these cell-membrane proteins in nuclei warrants further investigation. Although the antibodies used in this investigation were molecularly similar only E-cadherin, not occludin or zona occludens, was detected in extracellular coats. It is thus unlikely that this E-cadherin expression pattern is artifactual. Rather, its presence in embryonic coats as well as cell surfaces (as occurs in mouse) suggests that it may facilitate blastomere-zona adhesion well in advance of blastomere-blastomere adhesion.en_US
dc.language.isoen_USen_US
dc.publisherAcademic Press/Elsevier Scienceen_US
dc.identifier.doi10.1016/j.ydbio.2010.05.114en
dc.subject.departmentBiologyen_US
dc.titleExpression Pattern Of E-cad, Ocln And Zo-1 In Cleavage-stage Monodelphis Domestica Embryosen_US
dc.typearticleen_US
dc.identifier.journalDevelopmental Biologyen_US
dc.subject.keywordDevelopmental biologyen_US
dc.identifier.volume344en_US
dc.identifier.issue1en_US
dc.identifier.startpage75en_US
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