LacZ histochemistry and immunohistochemistry reveal ephrin-B ligand expression in the inner ear

Title:
LacZ histochemistry and immunohistochemistry reveal ephrin-B ligand expression in the inner ear
Authors:
Bianchi, Lynne M.; Dinsio, Kyl; Davoli, Katherine
Abstract:
Immunostaining in transgenic mice carrying the lac z gene can be used to map gene and protein distribution in a single tissue. In this study, we examined inner ears from ephrin-B3 homozygous and ephrin-B2 heterozygous mice. Ephrin-B3 lac z expression was limited in these mice. However, immunostaining revealed ephrin-B3 throughout cochlear and vestibular regions. Immunoreactivity was absent in ephrin-B3-homozygous null mutants, demonstrating the specificity of the antibody. Ephrin-B2 lac z reactivity was detected in a limited number of cells in cochlear and vestibular regions. Different immunostaining patterns were found with different antibodies. Comparison with lac z expression indicated which antibody was specific for the transmembrane-bound ephrin-B2 ligand.
Citation:
Bianchi, L.M., K. Dinsio, and N.W. Gale. 2002. "LacZ histochemistry and immunohistochemistry reveal ephrin-B ligand expression in the inner ear." Journal of Histochemistry and Cytochemistry 50: 1641-1645.
Publisher:
Histochemical Society
DATE ISSUED:
2002-12
Department:
Neuroscience
Type:
article
Additional Links:
http://lib.bioinfo.pl/paper:12486086
PERMANENT LINK:
http://hdl.handle.net/11282/309743

Full metadata record

DC FieldValue Language
dc.contributor.authorBianchi, Lynne M.en_US
dc.contributor.authorDinsio, Kylen_US
dc.contributor.authorDavoli, Katherineen_US
dc.date.accessioned2013-12-23T16:16:54Zen
dc.date.available2013-12-23T16:16:54Zen
dc.date.issued2002-12en
dc.identifier.citationBianchi, L.M., K. Dinsio, and N.W. Gale. 2002. "LacZ histochemistry and immunohistochemistry reveal ephrin-B ligand expression in the inner ear." Journal of Histochemistry and Cytochemistry 50: 1641-1645.en_US
dc.identifier.issn0022-1554en_US
dc.identifier.urihttp://hdl.handle.net/11282/309743en
dc.description.abstractImmunostaining in transgenic mice carrying the lac z gene can be used to map gene and protein distribution in a single tissue. In this study, we examined inner ears from ephrin-B3 homozygous and ephrin-B2 heterozygous mice. Ephrin-B3 lac z expression was limited in these mice. However, immunostaining revealed ephrin-B3 throughout cochlear and vestibular regions. Immunoreactivity was absent in ephrin-B3-homozygous null mutants, demonstrating the specificity of the antibody. Ephrin-B2 lac z reactivity was detected in a limited number of cells in cochlear and vestibular regions. Different immunostaining patterns were found with different antibodies. Comparison with lac z expression indicated which antibody was specific for the transmembrane-bound ephrin-B2 ligand.en_US
dc.language.isoen_USen_US
dc.publisherHistochemical Societyen_US
dc.relation.urlhttp://lib.bioinfo.pl/paper:12486086en_GB
dc.subject.departmentNeuroscienceen_US
dc.titleLacZ histochemistry and immunohistochemistry reveal ephrin-B ligand expression in the inner earen_US
dc.typearticleen_US
dc.identifier.journalJournal of Histochemistry and Cytochemistryen_US
dc.subject.keywordCochleaen_US
dc.subject.keywordVestibularen_US
dc.subject.keywordEphrin-B2en_US
dc.subject.keywordEphrin-B3en_US
dc.subject.keywordCTransgenic miceen_US
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