Embryonic inner ear cells reaggregate into specific patterns in vitro

Title:
Embryonic inner ear cells reaggregate into specific patterns in vitro
Authors:
Bianchi, Lynne M.; Person, Abigail, L.; Penney, Ellen B.
Abstract:
The sensory epithelia of the mammalian inner ear consist of a highly precise pattern of sensory hair cells and supporting cells. The mechanisms regulating this patterning are only beginning to be determined. The present study describes a method for culturing dissociated embryonic inner ear cells and the resulting patterning that occurs in these cultures. The results indicate that developing inner ear cells aggregate into precise patterns on a two-dimensional substrate, suggesting that intrinsic patterning mechanisms remain active in vitro. Using antibodies and scanning electron microscopy to detect hair cells and nonsensory cells, it was determined that only a subset of aggregates contained sensory hair cells. The hair cells were organized into specific patterns and surrounded by supporting cells, similar to the in vivo pattern. Additionally, hair cells increased their immunoreactivity and number of stereocilia over time, suggesting that hair cells continue to mature in vitro. Thus, the study reveals that the cells of the developing inner ear provide the necessary signals that direct sensory hair cells and supporting cells to reassociate into very precise patterns in vitro and that these patterns are reminiscent of the patterning that occurs in vivo.
Citation:
Bianchi, L.M., A.L. Person, and E.B. Penney. 2002. "Embryonic inner ear cells reaggregate into specific patterns in vitro." Journal of the Association for Research in Otolaryngology 3(4): 418-429.
Publisher:
Springer Verlag
DATE ISSUED:
2002-12
Department:
Neuroscience
Type:
article
PUBLISHED VERSION:
10.1007/s101620020042
PERMANENT LINK:
http://hdl.handle.net/11282/309742

Full metadata record

DC FieldValue Language
dc.contributor.authorBianchi, Lynne M.en_US
dc.contributor.authorPerson, Abigail, L.en_US
dc.contributor.authorPenney, Ellen B.en_US
dc.date.accessioned2013-12-23T16:16:52Zen
dc.date.available2013-12-23T16:16:52Zen
dc.date.issued2002-12en
dc.identifier.citationBianchi, L.M., A.L. Person, and E.B. Penney. 2002. "Embryonic inner ear cells reaggregate into specific patterns in vitro." Journal of the Association for Research in Otolaryngology 3(4): 418-429.en_US
dc.identifier.issn1525-3961en_US
dc.identifier.urihttp://hdl.handle.net/11282/309742en
dc.description.abstractThe sensory epithelia of the mammalian inner ear consist of a highly precise pattern of sensory hair cells and supporting cells. The mechanisms regulating this patterning are only beginning to be determined. The present study describes a method for culturing dissociated embryonic inner ear cells and the resulting patterning that occurs in these cultures. The results indicate that developing inner ear cells aggregate into precise patterns on a two-dimensional substrate, suggesting that intrinsic patterning mechanisms remain active in vitro. Using antibodies and scanning electron microscopy to detect hair cells and nonsensory cells, it was determined that only a subset of aggregates contained sensory hair cells. The hair cells were organized into specific patterns and surrounded by supporting cells, similar to the in vivo pattern. Additionally, hair cells increased their immunoreactivity and number of stereocilia over time, suggesting that hair cells continue to mature in vitro. Thus, the study reveals that the cells of the developing inner ear provide the necessary signals that direct sensory hair cells and supporting cells to reassociate into very precise patterns in vitro and that these patterns are reminiscent of the patterning that occurs in vivo.en_US
dc.language.isoen_USen_US
dc.publisherSpringer Verlagen_US
dc.identifier.doi10.1007/s101620020042en
dc.subject.departmentNeuroscienceen_US
dc.titleEmbryonic inner ear cells reaggregate into specific patterns in vitroen_US
dc.typearticleen_US
dc.identifier.journalJournal of the Association for Research in Otolaryngologyen_US
dc.subject.keywordHair cell developmenten_US
dc.subject.keywordCochleaen_US
dc.subject.keywordAuditoryen_US
dc.subject.keywordAuditoryen_US
dc.subject.keywordVestibularen_US
dc.subject.keywordOtocysten_US
dc.subject.keywordCalretininen_US
dc.subject.keywordMyosinen_US
dc.subject.keywordVIIaen_US
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