Microwave decalcification of human temporal bones

Title:
Microwave decalcification of human temporal bones
Authors:
Cunningham, Calhoun D., III; Schulte, Bradley A.; Bianchi, Lynne M.; Weber, Peter C.; Schmiedt, Barbara N.
Abstract:
Objectives/Hypothesis Morphological and immunohistochemical studies of human temporal bones are challenging as a result of difficulties in obtaining reliably fi-ed specimens and the lengthy time required for decalcification, typically 4 to 7 months. A novel method of processing human temporal bones using a microwave oven to accelerate decalcification is described. This procedure provides a rapid means of decalcifying temporal bones with good preservation of tissue morphology and antigenicity. Methods Human temporal bone specimens obtained at autopsy (n = 12, from specimens aged 43–91 y) were fixed within 6.5 hours of death by transtympanic perilymphatic perfusion of the inner ear. Decalcification was carried out using ethylenediaminetetra-acetic acid (EDTA) in a microwave oven and required only 3 to 6 weeks. Specimens were then dehydrated, embedded in paraffin, sectioned, and mounted on slides for morphological and immunohistochemical evaluation. Results Microscopic e-amination revealed no obvious artifacts attributable to the microwave decalcification process. The quality of morphological preservation was largely dependent on the postmortem fi-ation interval and adequacy of perilymphatic perfusion. Immunohistochemical analysis demonstrated strong positive staining for the enzyme Na,K-ATPase, an integral membrane protein. Conclusions This study demonstrates that microwave decalcification provides an efficient and reliable means of processing human temporal bones for histological and histochemical e-amination. Decalcification time is significantly reduced with no apparent adverse effects on structural preservation or antigenicity.
Citation:
Cunningham, C.D., B.A. Schulte, L.M. Bianchi, P.C. Weber, and B.N. Schmiedt. 2001. "Microwave decalcification of human temporal bones." Laryngoscope 111(2): 278-282.
Publisher:
John Wiley & Sons
DATE ISSUED:
2001-02
Department:
Neuroscience
Type:
article
PUBLISHED VERSION:
10.1097/00005537-200102000-00017
PERMANENT LINK:
http://hdl.handle.net/11282/309739

Full metadata record

DC FieldValue Language
dc.contributor.authorCunningham, Calhoun D., IIIen_US
dc.contributor.authorSchulte, Bradley A.en_US
dc.contributor.authorBianchi, Lynne M.en_US
dc.contributor.authorWeber, Peter C.en_US
dc.contributor.authorSchmiedt, Barbara N.en_US
dc.date.accessioned2013-12-23T16:16:41Zen
dc.date.available2013-12-23T16:16:41Zen
dc.date.issued2001-02en
dc.identifier.citationCunningham, C.D., B.A. Schulte, L.M. Bianchi, P.C. Weber, and B.N. Schmiedt. 2001. "Microwave decalcification of human temporal bones." Laryngoscope 111(2): 278-282.en_US
dc.identifier.issn0023-852Xen_US
dc.identifier.urihttp://hdl.handle.net/11282/309739en
dc.description.abstractObjectives/Hypothesis Morphological and immunohistochemical studies of human temporal bones are challenging as a result of difficulties in obtaining reliably fi-ed specimens and the lengthy time required for decalcification, typically 4 to 7 months. A novel method of processing human temporal bones using a microwave oven to accelerate decalcification is described. This procedure provides a rapid means of decalcifying temporal bones with good preservation of tissue morphology and antigenicity. Methods Human temporal bone specimens obtained at autopsy (n = 12, from specimens aged 43–91 y) were fixed within 6.5 hours of death by transtympanic perilymphatic perfusion of the inner ear. Decalcification was carried out using ethylenediaminetetra-acetic acid (EDTA) in a microwave oven and required only 3 to 6 weeks. Specimens were then dehydrated, embedded in paraffin, sectioned, and mounted on slides for morphological and immunohistochemical evaluation. Results Microscopic e-amination revealed no obvious artifacts attributable to the microwave decalcification process. The quality of morphological preservation was largely dependent on the postmortem fi-ation interval and adequacy of perilymphatic perfusion. Immunohistochemical analysis demonstrated strong positive staining for the enzyme Na,K-ATPase, an integral membrane protein. Conclusions This study demonstrates that microwave decalcification provides an efficient and reliable means of processing human temporal bones for histological and histochemical e-amination. Decalcification time is significantly reduced with no apparent adverse effects on structural preservation or antigenicity.en_US
dc.language.isoen_USen_US
dc.publisherJohn Wiley & Sonsen_US
dc.identifier.doi10.1097/00005537-200102000-00017en
dc.subject.departmentNeuroscienceen_US
dc.titleMicrowave decalcification of human temporal bonesen_US
dc.typearticleen_US
dc.identifier.journalLaryngoscopeen_US
dc.subject.keywordCochleaen_US
dc.subject.keywordInner earen_US
dc.subject.keywordNa,K-ATPaseen_US
dc.subject.keywordImmunohistochemistryen_US
dc.subject.keywordMorphologyen_US
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