Selective and transient expression of a native chondroitin sulfate epitope in Deiters’ cells, pillar cells, and the developing tectorial membrane

Title:
Selective and transient expression of a native chondroitin sulfate epitope in Deiters’ cells, pillar cells, and the developing tectorial membrane
Authors:
Bianchi, Lynne M.; Liu, H.; Krug, E. L.; Capehart, A. A.
Abstract:
The tectorial membrane (TM) is an acellular connective tissue overlying the sensory hair cells of the organ of Corti. Association of the tectorial membrane with the stereocilia of the sensory hair cells is necessary for proper auditory function. During development, the mature tectorial membrane is thought to arise by fusion of a “major” and “minor” tectorial membrane (Lim, Hear Res 1986;22:117–146). Several proteins and glycoconjugates have been detected in the developing TM; however, the specific molecules which mediate fusion of the two components of the TM have not been identified. In the present study, a novel monoclonal antibody (TC2) that recognizes a native epitope on glycosaminoglycans enriched in chondroitin-4-sulfate revealed a transient and restricted expression in the developing gerbil TM. The localization patterns suggest that Deiters’ and pillar cells secrete a TC2-positive matrix prior to birth that later becomes incorporated into the marginal band and superior layer (cover net) of the TM. The developmental timecourse and patterns of TC2 reactivity suggest that this molecule may play a critical role in the fusion of the minor TM with the major TM.
Citation:
Bianchi, L.M., H. Liu, E.L. Krug, and A.A. Capehart. 1999. "Selective and transient expression of a native chondroitin sulfate epitope in Deiters’ cells, pillar cells, and the developing tectorial membrane." Anatomical Record 256(1): 64-71.
Publisher:
John Wiley & Sons
DATE ISSUED:
1999-9-1
Department:
Neuroscience
Type:
article
Additional Links:
http://www.ncbi.nlm.nih.gov/pubmed/10456986
PERMANENT LINK:
http://hdl.handle.net/11282/309736

Full metadata record

DC FieldValue Language
dc.contributor.authorBianchi, Lynne M.en_US
dc.contributor.authorLiu, H.en_US
dc.contributor.authorKrug, E. L.en_US
dc.contributor.authorCapehart, A. A.en_US
dc.date.accessioned2013-12-23T16:16:37Zen
dc.date.available2013-12-23T16:16:37Zen
dc.date.issued1999-9-1en
dc.identifier.citationBianchi, L.M., H. Liu, E.L. Krug, and A.A. Capehart. 1999. "Selective and transient expression of a native chondroitin sulfate epitope in Deiters’ cells, pillar cells, and the developing tectorial membrane." Anatomical Record 256(1): 64-71.en_US
dc.identifier.issn0003-276Xen_US
dc.identifier.urihttp://hdl.handle.net/11282/309736en
dc.description.abstractThe tectorial membrane (TM) is an acellular connective tissue overlying the sensory hair cells of the organ of Corti. Association of the tectorial membrane with the stereocilia of the sensory hair cells is necessary for proper auditory function. During development, the mature tectorial membrane is thought to arise by fusion of a “major” and “minor” tectorial membrane (Lim, Hear Res 1986;22:117–146). Several proteins and glycoconjugates have been detected in the developing TM; however, the specific molecules which mediate fusion of the two components of the TM have not been identified. In the present study, a novel monoclonal antibody (TC2) that recognizes a native epitope on glycosaminoglycans enriched in chondroitin-4-sulfate revealed a transient and restricted expression in the developing gerbil TM. The localization patterns suggest that Deiters’ and pillar cells secrete a TC2-positive matrix prior to birth that later becomes incorporated into the marginal band and superior layer (cover net) of the TM. The developmental timecourse and patterns of TC2 reactivity suggest that this molecule may play a critical role in the fusion of the minor TM with the major TM.en_US
dc.language.isoen_USen_US
dc.publisherJohn Wiley & Sonsen_US
dc.relation.urlhttp://www.ncbi.nlm.nih.gov/pubmed/10456986en_US
dc.subject.departmentNeuroscienceen_US
dc.titleSelective and transient expression of a native chondroitin sulfate epitope in Deiters’ cells, pillar cells, and the developing tectorial membraneen_US
dc.typearticleen_US
dc.identifier.journalAnatomical Recorden_US
dc.subject.keywordCochleaen_US
dc.subject.keywordOrgan of Cortien_US
dc.subject.keywordGerbilen_US
dc.subject.keywordGlycosaminoglycanen_US
dc.subject.keywordChondroitin-4-sulfateen_US
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