Developmental changes in growth factors released by the embryonic inner ear

Title:
Developmental changes in growth factors released by the embryonic inner ear
Authors:
Bianchi, Lynne M.; Dolnick, R.; Medd, Amy M.; Cohan, Christopher S.
Abstract:
Recent studies have demonstrated a role for neurotrophins in regulating the survival of developing auditory and vestibular neurons. However, the developmental time-course for neurotrophin production and release by inner ear tissues has not been defined. In the present study, neurotrophin-like activity was evaluated from culture medium conditioned by early- or midembryonic stage inner ears. Examination of the proteinaceous properties of conditioned medium revealed a developmental change in growth factor release by the inner ear. Neurotrophin-like molecules were not detected in medium conditioned by early stage otocysts. In contrast, neurotrophin-like bioactivity was detected in medium conditioned by middevelopmental stage inner ears. Western blot analysis revealed that NT-3 was released by the rat inner ear at midstages of inner ear development. ELISA measurements revealed that both NT-3 and BDNF are produced by the middevelopmental stage inner ear, and that NT-3 protein levels are higher than BDNF levels. These results suggest that there are developmental changes in the release of growth factors by the inner ear.
Citation:
Bianchi, L.M., R. Dolnick, A.M. Medd, and C.S. Cohan. 1998. "Developmental changes in growth factors released by the embryonic inner ear." Experimental Neurology 150(1): 98-106.
Publisher:
Elsevier
DATE ISSUED:
1998-03
Department:
Neuroscience
Type:
article
PUBLISHED VERSION:
10.1006/exnr.1997.6774
PERMANENT LINK:
http://hdl.handle.net/11282/309734

Full metadata record

DC FieldValue Language
dc.contributor.authorBianchi, Lynne M.en_US
dc.contributor.authorDolnick, R.en_US
dc.contributor.authorMedd, Amy M.en_US
dc.contributor.authorCohan, Christopher S.en_US
dc.date.accessioned2013-12-23T16:16:34Zen
dc.date.available2013-12-23T16:16:34Zen
dc.date.issued1998-03en
dc.identifier.citationBianchi, L.M., R. Dolnick, A.M. Medd, and C.S. Cohan. 1998. "Developmental changes in growth factors released by the embryonic inner ear." Experimental Neurology 150(1): 98-106.en_US
dc.identifier.issn0014-4886en_US
dc.identifier.urihttp://hdl.handle.net/11282/309734en
dc.description.abstractRecent studies have demonstrated a role for neurotrophins in regulating the survival of developing auditory and vestibular neurons. However, the developmental time-course for neurotrophin production and release by inner ear tissues has not been defined. In the present study, neurotrophin-like activity was evaluated from culture medium conditioned by early- or midembryonic stage inner ears. Examination of the proteinaceous properties of conditioned medium revealed a developmental change in growth factor release by the inner ear. Neurotrophin-like molecules were not detected in medium conditioned by early stage otocysts. In contrast, neurotrophin-like bioactivity was detected in medium conditioned by middevelopmental stage inner ears. Western blot analysis revealed that NT-3 was released by the rat inner ear at midstages of inner ear development. ELISA measurements revealed that both NT-3 and BDNF are produced by the middevelopmental stage inner ear, and that NT-3 protein levels are higher than BDNF levels. These results suggest that there are developmental changes in the release of growth factors by the inner ear.en_US
dc.language.isoen_USen_US
dc.publisherElsevieren_US
dc.identifier.doi10.1006/exnr.1997.6774en_US
dc.subject.departmentNeuroscienceen_US
dc.titleDevelopmental changes in growth factors released by the embryonic inner earen_US
dc.typearticleen_US
dc.identifier.journalExperimental Neurologyen_US
dc.subject.keywordEmbryonic inner earen_US
dc.subject.keywordOtocysten_US
dc.subject.keywordGrowth factorsen_US
dc.subject.keywordNeurotrophinen_US
dc.subject.keywordStatoacoustic ganglionen_US
dc.subject.keywordNeuronal survivalen_US
dc.subject.keywordAuditoryen_US
dc.subject.keywordVestibularen_US
dc.identifier.volume150en_US
dc.identifier.issue1en_US
dc.identifier.startpage98en_US
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