Effects of the neurotrophins and CNTF on developing statoacoustic neurons: Comparison with an otocyst-derived factor

Title:
Effects of the neurotrophins and CNTF on developing statoacoustic neurons: Comparison with an otocyst-derived factor
Authors:
Bianchi, Lynne M.; Cohan, Christopher S.
Abstract:
During the early stages of auditory development, the inner ear (otocyst) releases an unidentified, diffusible factor that promotes neurite outgrowth from the associated statoacoustic ganglia (SAG). Using a variety of criteria, the present study compared the neurite- and survival-promoting properties of this otocyst-derived factor (ODF) to the neurotrophins NGF, BDNF, NT-3, and NT-4 and ciliary neurotrophic factor (CNTF). Ganglia known to respond to specific growth factors were cultured in the presence of ODF. ODF failed to promote neurite outgrowth from trigeminal, ciliary, sympathetic, or dorsal root ganglia, suggesting that ODF may have properties different from other identified growth factors. In complementary experiments, SAG explants were cultured in ODF, the neurotrophins, and CNTF. The extent of outgrowth was greatest in the presence of ODF and CNTF, with the neurotrophins having little effect. In neuron-enriched, dissociated cell cultures, ODF promoted both survival and outgrowth of SAG neurons. However, neither the neurotrophins nor CNTF, alone or in combination, promoted the survival or outgrowth of dissociated SAG neurons. Thus, the outgrowth seen in the explant cultures appears to be due to indirect effects via the ganglionic nonneuronal cells. The addition of anti-NGF antisera failed to block the activity of chick, rat, or mouse ODF, further indicating that NGF is not the primary component of ODF. Together, the results of this study indicate that the properties of the ODF are not mimicked by the neurotrophins or CNTF.
Citation:
Bianchi, L.M., and C.S. Cohan. 1993. "Effects of the neurotrophins and CNTF on developing statoacoustic neurons: Comparison with an otocyst-derived factor." Developmental Biology 159(1): 353-365.
Publisher:
Academic Press
DATE ISSUED:
1993-09
Department:
Neuroscience
Type:
article
PUBLISHED VERSION:
10.1006/dbio.1993.1247
PERMANENT LINK:
http://hdl.handle.net/11282/309732

Full metadata record

DC FieldValue Language
dc.contributor.authorBianchi, Lynne M.en_US
dc.contributor.authorCohan, Christopher S.en_US
dc.date.accessioned2013-12-23T16:16:32Zen
dc.date.available2013-12-23T16:16:32Zen
dc.date.issued1993-09en
dc.identifier.citationBianchi, L.M., and C.S. Cohan. 1993. "Effects of the neurotrophins and CNTF on developing statoacoustic neurons: Comparison with an otocyst-derived factor." Developmental Biology 159(1): 353-365.en_US
dc.identifier.issn0012-1606en_US
dc.identifier.urihttp://hdl.handle.net/11282/309732en
dc.description.abstractDuring the early stages of auditory development, the inner ear (otocyst) releases an unidentified, diffusible factor that promotes neurite outgrowth from the associated statoacoustic ganglia (SAG). Using a variety of criteria, the present study compared the neurite- and survival-promoting properties of this otocyst-derived factor (ODF) to the neurotrophins NGF, BDNF, NT-3, and NT-4 and ciliary neurotrophic factor (CNTF). Ganglia known to respond to specific growth factors were cultured in the presence of ODF. ODF failed to promote neurite outgrowth from trigeminal, ciliary, sympathetic, or dorsal root ganglia, suggesting that ODF may have properties different from other identified growth factors. In complementary experiments, SAG explants were cultured in ODF, the neurotrophins, and CNTF. The extent of outgrowth was greatest in the presence of ODF and CNTF, with the neurotrophins having little effect. In neuron-enriched, dissociated cell cultures, ODF promoted both survival and outgrowth of SAG neurons. However, neither the neurotrophins nor CNTF, alone or in combination, promoted the survival or outgrowth of dissociated SAG neurons. Thus, the outgrowth seen in the explant cultures appears to be due to indirect effects via the ganglionic nonneuronal cells. The addition of anti-NGF antisera failed to block the activity of chick, rat, or mouse ODF, further indicating that NGF is not the primary component of ODF. Together, the results of this study indicate that the properties of the ODF are not mimicked by the neurotrophins or CNTF.en_US
dc.language.isoen_USen_US
dc.publisherAcademic Pressen_US
dc.identifier.doi10.1006/dbio.1993.1247en_US
dc.subject.departmentNeuroscienceen_US
dc.titleEffects of the neurotrophins and CNTF on developing statoacoustic neurons: Comparison with an otocyst-derived factoren_US
dc.typearticleen_US
dc.identifier.journalDevelopmental Biologyen_US
dc.identifier.volume159en_US
dc.identifier.issue1en_US
dc.identifier.startpage353en_US
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