Embryonic inner ear cells use migratory mechanisms to establish cell patterns in vitro

Title:
Embryonic inner ear cells use migratory mechanisms to establish cell patterns in vitro
Authors:
Bianchi, Lynne M.; Huri, Daniel; White, Ian O.
Abstract:
The hair cells of the sensory epithelium in the inner ear are among the most precisely organized cells in vertebrates. The mechanisms that lead to this orderly arrangement are only beginning to be understood. It has been suggested that hair cells use migratory mechanisms to help achieve their final position in the organ of Corti. The small size and complex organization of the intact inner ear have made it difficult to monitor changes in hair cell location over time in vivo. In the present study, an established in vitro assay of dissociated, embryonic inner ear cells was used to monitor how hair cells reorganize over time. The hair cell specific marker myosin-VI demonstrated that hair cell precursors from both cochlear and vestibular regions reorganized into specific patterns between 3–24 hr in vitro. In contrast to the unlabeled cells, the myosin-VI-positive cells extended processes while establishing the hair cell patterning within an aggregate. These studies support the hypothesis that hair cell precursors actively migrate to help achieve final patterning within the inner ear sensory epithelium.
Citation:
Bianchi, L.M., D. Huri, and I.O. White. 2006. "Embryonic inner ear cells use migratory mechanisms to establish cell patterns in vitro." Journal of Neuroscience Research 83(2): 191-198.
Publisher:
Wiley-Blackwell
DATE ISSUED:
2006-02-01
Department:
Neuroscience
Type:
article
PUBLISHED VERSION:
10.1002/jnr.20710
PERMANENT LINK:
http://hdl.handle.net/11282/309730

Full metadata record

DC FieldValue Language
dc.contributor.authorBianchi, Lynne M.en_US
dc.contributor.authorHuri, Danielen_US
dc.contributor.authorWhite, Ian O.en_US
dc.date.accessioned2013-12-23T16:16:30Zen
dc.date.available2013-12-23T16:16:30Zen
dc.date.issued2006-02-01en
dc.identifier.citationBianchi, L.M., D. Huri, and I.O. White. 2006. "Embryonic inner ear cells use migratory mechanisms to establish cell patterns in vitro." Journal of Neuroscience Research 83(2): 191-198.en_US
dc.identifier.issn0360-4012en_US
dc.identifier.urihttp://hdl.handle.net/11282/309730en
dc.description.abstractThe hair cells of the sensory epithelium in the inner ear are among the most precisely organized cells in vertebrates. The mechanisms that lead to this orderly arrangement are only beginning to be understood. It has been suggested that hair cells use migratory mechanisms to help achieve their final position in the organ of Corti. The small size and complex organization of the intact inner ear have made it difficult to monitor changes in hair cell location over time in vivo. In the present study, an established in vitro assay of dissociated, embryonic inner ear cells was used to monitor how hair cells reorganize over time. The hair cell specific marker myosin-VI demonstrated that hair cell precursors from both cochlear and vestibular regions reorganized into specific patterns between 3–24 hr in vitro. In contrast to the unlabeled cells, the myosin-VI-positive cells extended processes while establishing the hair cell patterning within an aggregate. These studies support the hypothesis that hair cell precursors actively migrate to help achieve final patterning within the inner ear sensory epithelium.en_US
dc.language.isoen_USen_US
dc.publisherWiley-Blackwellen_US
dc.identifier.doi10.1002/jnr.20710en
dc.subject.departmentNeuroscienceen_US
dc.titleEmbryonic inner ear cells use migratory mechanisms to establish cell patterns in vitroen_US
dc.typearticleen_US
dc.identifier.journalJournal of Neuroscience Researchen_US
dc.subject.keywordCochleaen_US
dc.subject.keywordHair cellen_US
dc.subject.keywordVestibularen_US
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