Cue-induced conditioned activity does not incubate but is mediated by the basolateral amygdala

Title:
Cue-induced conditioned activity does not incubate but is mediated by the basolateral amygdala
Authors:
Diehl, Geoffrey W.; Wachtel, Jonathan M.; Paine, Tracie A.
Abstract:
Re-exposure to drug-associated cues causes significant drug craving in recovering addicts, which may precipitate relapse. In animal models of craving, drug-seeking responses for contingent delivery of drug-associated cues sensitizes or “incubates” across drug withdrawal. To date there is limited evidence supporting an incubation effect for behaviors mediated by non-contingent presentation of drug-associated cues. Here we used a model of cue-induced conditioned activity to determine if the conditioned locomotor response to a non-contingent presentation of a drug-associated cue sensitizes across drug withdrawal. In addition, because cue-induced drug-seeking responses are mediated by the rostral basolateral amygdala (BLA), we investigated whether this structure is critical for the expression of cue-induced conditioned activity. A conditioned association between cocaine (15 mg/kg) and a compound discrete cue (flashing bicycle light + a metronome) was established over 12 conditioning sessions in male Sprague–Dawley rats. In experiment 1, cue-induced conditioned activity was assessed on 3 occasions: 3, 14 and 28 days following the final drug–cue conditioning session. Cocaine-conditioned rats demonstrated reliable cue-induced conditioned activity across all 3 test sessions, however there was no evidence of an incubation effect. To determine whether repeated testing prevented the observation of an incubation effect, rats in experiment 2 were tested either 3 days or 28 days following conditioning; again no incubation effect was observed. In experiment 3, either saline or the GABAA receptor agonist muscimol was infused prior to testing. Intra-BLA infusions of muscimol prevented the expression of cue-induced conditioned activity. These data support the role of the rBLA in mediating conditioned responses to drug-associated cues. The failure to observe an incubation effect for cue-induced conditioned activity may point to a fundamental difference in the manner by which contingent and non-contingent presentations of drug-associated cues influence behavior.
Citation:
Deihl, Geoffrey W., J.M. Wachtel1, and T.A. Paine. 2013. "Cue-induced conditioned activity does not incubate but is mediated by the basolateral amygdala." Pharmacology Biochemistry and Behavior 104: 69-79.
Publisher:
Elsevier
DATE ISSUED:
2013-03
Department:
Neuroscience
Type:
article
PUBLISHED VERSION:
10.1016/j.pbb.2013.01.003
PERMANENT LINK:
http://hdl.handle.net/11282/309709

Full metadata record

DC FieldValue Language
dc.contributor.authorDiehl, Geoffrey W.en_US
dc.contributor.authorWachtel, Jonathan M.en_US
dc.contributor.authorPaine, Tracie A.en_US
dc.date.accessioned2013-12-23T16:16:01Zen
dc.date.available2013-12-23T16:16:01Zen
dc.date.issued2013-03en
dc.identifier.citationDeihl, Geoffrey W., J.M. Wachtel1, and T.A. Paine. 2013. "Cue-induced conditioned activity does not incubate but is mediated by the basolateral amygdala." Pharmacology Biochemistry and Behavior 104: 69-79.en_US
dc.identifier.issn0091-3057en_US
dc.identifier.urihttp://hdl.handle.net/11282/309709en
dc.description.abstractRe-exposure to drug-associated cues causes significant drug craving in recovering addicts, which may precipitate relapse. In animal models of craving, drug-seeking responses for contingent delivery of drug-associated cues sensitizes or “incubates” across drug withdrawal. To date there is limited evidence supporting an incubation effect for behaviors mediated by non-contingent presentation of drug-associated cues. Here we used a model of cue-induced conditioned activity to determine if the conditioned locomotor response to a non-contingent presentation of a drug-associated cue sensitizes across drug withdrawal. In addition, because cue-induced drug-seeking responses are mediated by the rostral basolateral amygdala (BLA), we investigated whether this structure is critical for the expression of cue-induced conditioned activity. A conditioned association between cocaine (15 mg/kg) and a compound discrete cue (flashing bicycle light + a metronome) was established over 12 conditioning sessions in male Sprague–Dawley rats. In experiment 1, cue-induced conditioned activity was assessed on 3 occasions: 3, 14 and 28 days following the final drug–cue conditioning session. Cocaine-conditioned rats demonstrated reliable cue-induced conditioned activity across all 3 test sessions, however there was no evidence of an incubation effect. To determine whether repeated testing prevented the observation of an incubation effect, rats in experiment 2 were tested either 3 days or 28 days following conditioning; again no incubation effect was observed. In experiment 3, either saline or the GABAA receptor agonist muscimol was infused prior to testing. Intra-BLA infusions of muscimol prevented the expression of cue-induced conditioned activity. These data support the role of the rBLA in mediating conditioned responses to drug-associated cues. The failure to observe an incubation effect for cue-induced conditioned activity may point to a fundamental difference in the manner by which contingent and non-contingent presentations of drug-associated cues influence behavior.en_US
dc.language.isoen_USen_US
dc.publisherElsevieren_US
dc.identifier.doi10.1016/j.pbb.2013.01.003en
dc.subject.departmentNeuroscienceen_US
dc.titleCue-induced conditioned activity does not incubate but is mediated by the basolateral amygdalaen_US
dc.typearticleen_US
dc.identifier.journalPharmacology Biochemistry and Behavioren_US
dc.subject.keywordCocaineen_US
dc.subject.keywordPavlovian conditioningen_US
dc.subject.keywordRelapseen_US
dc.subject.keywordIncubation effecten_US
dc.subject.keywordBasolateral amygdalaen_US
dc.subject.keywordConditioned activityen_US
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