Immortalized Mouse Inner Ear Cell Lines Demonstrate a Role for Chemokines in Promoting the Growth of Developing Statoacoustic Ganglion Neurons

Title:
Immortalized Mouse Inner Ear Cell Lines Demonstrate a Role for Chemokines in Promoting the Growth of Developing Statoacoustic Ganglion Neurons
Authors:
Bianchi, Lynne M.; Daruwalla, Zeeba; Roth, Therese M.; Attia, Naweah P.; Lukacs, Nicholas W.; Richards, Ayo-Lynn; White, Ian O.; Allen, Susan J.; Barald, Kate F.
Abstract:
The target-derived factors necessary for promoting initial outgrowth from the statoacoustic ganglion (SAG) to the inner ear have not been fully characterized. In the present study, conditioned medium from embryonic Immortomouse inner ear cell lines that maintain many characteristics of developing inner ear sensory epithelia were screened for neurite-promoting activity. Conditioned medium found to be positive for promoting SAG neurite outgrowth and neuronal survival was then tested for the presence of chemokines, molecules that have not previously been investigated for promoting SAG outgrowth. One candidate molecule, monocyte chemotactic protein 1 (MCP-1), was detected in the conditioned medium and subsequently localized to mouse hair cells by immunocytochemistry. In vitro studies demonstrated that function-blocking MCP-1 antibodies decreased the amount of SAG neurite outgrowth induced by the conditioned medium and that subsequent addition of MCP-1 protein was able to promote outgrowth when added to the antibody-treated conditioned medium. The use of the Immortomouse cell lines proved valuable in identifying this candidate cofactor that promotes outgrowth of early-stage SAG nerve fibers and is expressed in embryonic hair cells.
Citation:
Bianchi, L.M., Z. Daruwalla, T.M. Roth, N.P. Attia, N.W. Lukacs, A-L. Richards, I.O. White, S.J. Allen, and K.F. Barald. 2005. "Immortalized mouse inner ear cell lines demonstrate a role for chemokines in promoting the growth of developing statoacoustic ganglion neurons." Journal of the Association for Research in Otolaryngology 6(4): 355–367.
Publisher:
Springer Verlag
DATE ISSUED:
2005-12
Department:
Neuroscience
Type:
Article
PUBLISHED VERSION:
10.1007/s10162-005-0013-8
Additional Links:
http://link.springer.com/10.1007/s10162-005-0013-8
PERMANENT LINK:
http://hdl.handle.net/11282/294170

Full metadata record

DC FieldValue Language
dc.contributor.authorBianchi, Lynne M.en_US
dc.contributor.authorDaruwalla, Zeebaen_US
dc.contributor.authorRoth, Therese M.en_US
dc.contributor.authorAttia, Naweah P.en_US
dc.contributor.authorLukacs, Nicholas W.en_US
dc.contributor.authorRichards, Ayo-Lynnen_US
dc.contributor.authorWhite, Ian O.en_US
dc.contributor.authorAllen, Susan J.en_US
dc.contributor.authorBarald, Kate F.en_US
dc.date.accessioned2013-06-18T15:02:11Zen
dc.date.available2013-06-18T15:02:11Zen
dc.date.issued2005-12en
dc.identifier.citationBianchi, L.M., Z. Daruwalla, T.M. Roth, N.P. Attia, N.W. Lukacs, A-L. Richards, I.O. White, S.J. Allen, and K.F. Barald. 2005. "Immortalized mouse inner ear cell lines demonstrate a role for chemokines in promoting the growth of developing statoacoustic ganglion neurons." Journal of the Association for Research in Otolaryngology 6(4): 355–367.en_US
dc.identifier.issn1525-3961en_US
dc.identifier.urihttp://hdl.handle.net/11282/294170en
dc.description.abstractThe target-derived factors necessary for promoting initial outgrowth from the statoacoustic ganglion (SAG) to the inner ear have not been fully characterized. In the present study, conditioned medium from embryonic Immortomouse inner ear cell lines that maintain many characteristics of developing inner ear sensory epithelia were screened for neurite-promoting activity. Conditioned medium found to be positive for promoting SAG neurite outgrowth and neuronal survival was then tested for the presence of chemokines, molecules that have not previously been investigated for promoting SAG outgrowth. One candidate molecule, monocyte chemotactic protein 1 (MCP-1), was detected in the conditioned medium and subsequently localized to mouse hair cells by immunocytochemistry. In vitro studies demonstrated that function-blocking MCP-1 antibodies decreased the amount of SAG neurite outgrowth induced by the conditioned medium and that subsequent addition of MCP-1 protein was able to promote outgrowth when added to the antibody-treated conditioned medium. The use of the Immortomouse cell lines proved valuable in identifying this candidate cofactor that promotes outgrowth of early-stage SAG nerve fibers and is expressed in embryonic hair cells.en_US
dc.language.isoen_USen_US
dc.publisherSpringer Verlagen_US
dc.identifier.doi10.1007/s10162-005-0013-8en
dc.relation.urlhttp://link.springer.com/10.1007/s10162-005-0013-8en_GB
dc.subject.departmentNeuroscienceen_US
dc.titleImmortalized Mouse Inner Ear Cell Lines Demonstrate a Role for Chemokines in Promoting the Growth of Developing Statoacoustic Ganglion Neuronsen_US
dc.typeArticleen_US
dc.identifier.journalJournal of the Association for Research in Otolaryngologyen_US
dc.rightsArchived with thanks to Journal of the Association for Research in Otolaryngologyen_GB
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